Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | catalysis declines due to suicide inactivation; catalysis declines due to suicide inactivation. Following column chromatography, e.g. ion exchange or zinc-affinity chromatography, hydroperoxide isomerase activity is often decreased and sometimes even undetectable by TLC, whereas 8-dioxygenase activity is always present. However, storage of the enzyme for a few days on ice often restores the hydroperoxide isomerase and appeares to increase the 7,8-linoleate diol synthase activity as well | Gaeumannomyces graminis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Gaeumannomyces graminis | - |
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | Gaeumannomyces graminis var. graminis | - |
(8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaeumannomyces graminis | - |
- |
- |
Gaeumannomyces graminis | Q9UUS2 | - |
- |
Gaeumannomyces graminis var. graminis | - |
- |
- |
Gaeumannomyces graminis var. graminis | Q9UUS2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gaeumannomyces graminis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Gaeumannomyces graminis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Gaeumannomyces graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group | Gaeumannomyces graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | bifunctional enzyme with linoleic acid 8R-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group | Gaeumannomyces graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | - |
Gaeumannomyces graminis var. graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group | Gaeumannomyces graminis var. graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? | |
linoleate + O2 | bifunctional enzyme with linoleic acid 8R-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group | Gaeumannomyces graminis var. graminis | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | ferric hemeprotein | Gaeumannomyces graminis |