Crystallization (Comment) | Organism |
---|---|
hanging drop method | Cupriavidus metallidurans |
Protein Variants | Comment | Organism |
---|---|---|
E110A | kcat/Km is 954fold lower than wild-type enzyme | Cupriavidus metallidurans |
R47A | kcat/Km is 7440fold lower than wild-type enzyme. Mutant enzyme shows substrate inhibition | Cupriavidus metallidurans |
R99A | kcat/Km is 22320fold lower than wild-type enzyme | Cupriavidus metallidurans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Chloro-3-hydroxyanthranilate | - |
Cupriavidus metallidurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme E110A | Cupriavidus metallidurans | |
0.0224 | - |
3-Hydroxyanthranilate | pH 7.2, wild-type enzyme | Cupriavidus metallidurans | |
0.147 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme R47A | Cupriavidus metallidurans | |
0.872 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme R99A | Cupriavidus metallidurans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | each monomer contains two iron binding sites. The catalytic iron is buried deep inside the beta-barrel with His51, Glu57, and His95 serving as ligands. The other iron site forms an FeS4 center close to the solvent surface in which the sulfur atoms are provided by Cys125, Cys128, Cys162, and Cys165. The two iron sites are separated by 24 A | Cupriavidus metallidurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus metallidurans | - |
recombinantly expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus metallidurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyanthranilate + O2 | - |
Cupriavidus metallidurans | 2-amino-3-carboxymuconate semialdehyde | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme R99A | Cupriavidus metallidurans | |
0.012 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme E110A | Cupriavidus metallidurans | |
0.022 | - |
3-Hydroxyanthranilate | pH 7.2, mutant enzyme R47A | Cupriavidus metallidurans | |
25 | - |
3-Hydroxyanthranilate | pH 7.2, wild-type enzyme | Cupriavidus metallidurans |