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Literature summary for 1.13.11.6 extracted from
- Purification and properties of 3-hydroxyanthranilic acid oxidase (1961), J. Biol. Chem., 236, 3076-3082.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
3-hydroxyanthranilic acid | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | the most effective salt, the optimal concentration is 0.035 M | Bos taurus | |
| Fe2+ | solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form | Bos taurus | |
| HCl | during purification of enzyme treatment with acid was used | Bos taurus | |
| MgCl2 | - |
Bos taurus | |
| NaCl | - |
Bos taurus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | drastic change in the Km of the enzyme in the presence of dimethylglutarate buffer | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
reaches a maximum after 10 min at acid pH | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, in 0.01 M Tris buffer, pH 7.0, 4 days, no loss of activity | Bos taurus |
| 4°C, overnight, about 75% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxyanthranilate + O2 | - |
Bos taurus | 2-amino-3-carboxymuconate semialdehyde | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
activation by heating at 55°C for 5 min | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
at pH 3.4: the enzyme is in a form which can bind substrate but is enzymatically inactive, at pH 6.5: active form of enzyme | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
4°C, half-life: 3 days | Bos taurus |
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Release 2023.1 (January 2023)
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