Cloned (Comment) | Organism |
---|---|
gene AgLOX1, cloned from chitosan-induced seedling, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Cynanchum gracillimum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
x * 98000, about, sequence calculation, x * 110700, recombinant His-tagged enzyme, SDS-PAGE | Cynanchum gracillimum |
110700 | - |
x * 98000, about, sequence calculation, x * 110700, recombinant His-tagged enzyme, SDS-PAGE | Cynanchum gracillimum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cynanchum gracillimum | in chitosan-treated Adelostemma gracillimum seedlings 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, accumulate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cynanchum gracillimum | Q4FCM5 | collected in Shangrila County, Yunnan Province, China, gene AgLOX1 | - |
Purification (Comment) | Organism |
---|---|
recombinnat enzyme partially from Escherichia coli by nickel affinity chromatography | Cynanchum gracillimum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Cynanchum gracillimum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in chitosan-treated Adelostemma gracillimum seedlings 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, accumulate | Cynanchum gracillimum | ? | - |
? | |
additional information | the enzyme converted linoleic and linolenic acids almost exclusively to their 9-hydroperoxides | Cynanchum gracillimum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98000, about, sequence calculation, x * 110700, recombinant His-tagged enzyme, SDS-PAGE | Cynanchum gracillimum |
Synonyms | Comment | Organism |
---|---|---|
9-lipoxygenase | - |
Cynanchum gracillimum |
9-LOX | - |
Cynanchum gracillimum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Cynanchum gracillimum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Cynanchum gracillimum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cynanchum gracillimum | gene AgLOX1 sequence calculation | - |
5.94 |
Organism | Comment | Expression |
---|---|---|
Cynanchum gracillimum | the enzyme is induced by low molecular weight chitosan, expression pattern, overview | up |
General Information | Comment | Organism |
---|---|---|
physiological function | chitosan-induced AgLOX1 encodes a 9-lipoxygenase potentially involved in the defense response through 9-LOX pathway leading to biosynthesis of antimicrobial compounds in Adelostemma gracillimum seedlings | Cynanchum gracillimum |