Application | Comment | Organism |
---|---|---|
synthesis | the yeast expressed Nb-9-LOX can be used to produce C9-aldehydes on a large scale in combination with a HPL gene with 9-hydroperoxide lyase function, or to effectively produce 9-hydroxy-10(E),12(Z)-octadecadienoic acid in a biocatalytic process in combination with cysteine as a mild reducing agent | Nicotiana benthamiana |
Cloned (Comment) | Organism |
---|---|
gene Nb-9-LOX, DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae, quantitative realtime PCR exxpression analysis | Nicotiana benthamiana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
linoleate | pH 6.0, 35°C, recombinant enzyme | Nicotiana benthamiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97400 | - |
x * 97400, about, sequence calculation | Nicotiana benthamiana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | Nicotiana benthamiana | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
additional information | Nicotiana benthamiana | Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana benthamiana | - |
gene Nb-9-LOX | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana benthamiana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Nicotiana benthamiana | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
linoleate + O2 | regiospecificity of Nb-9-LOX, overview | Nicotiana benthamiana | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
? | |
additional information | Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function | Nicotiana benthamiana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 97400, about, sequence calculation | Nicotiana benthamiana |
Synonyms | Comment | Organism |
---|---|---|
9-LOX | - |
Nicotiana benthamiana |
Nb-9-LOX | - |
Nicotiana benthamiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Nicotiana benthamiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Nicotiana benthamiana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nicotiana benthamiana | sequence calculation | - |
5.52 |
Organism | Comment | Expression |
---|---|---|
Nicotiana benthamiana | the enzyme expression is highly induced by agroinfiltration of the plant leaves using a tobacco mosaic virus based vector system | up |