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Literature summary for 1.13.11.58 extracted from

  • Huang, F.C.; Schwab, W.
    Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamiana for biotechnological application (2011), BMC Biotechnol., 11, 30.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the yeast expressed Nb-9-LOX can be used to produce C9-aldehydes on a large scale in combination with a HPL gene with 9-hydroperoxide lyase function, or to effectively produce 9-hydroxy-10(E),12(Z)-octadecadienoic acid in a biocatalytic process in combination with cysteine as a mild reducing agent Nicotiana benthamiana

Cloned(Commentary)

Cloned (Comment) Organism
gene Nb-9-LOX, DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae, quantitative realtime PCR exxpression analysis Nicotiana benthamiana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
linoleate pH 6.0, 35°C, recombinant enzyme Nicotiana benthamiana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97400
-
x * 97400, about, sequence calculation Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Nicotiana benthamiana
-
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
?
additional information Nicotiana benthamiana Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function ?
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana benthamiana
-
gene Nb-9-LOX
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nicotiana benthamiana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
Nicotiana benthamiana (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
?
linoleate + O2 regiospecificity of Nb-9-LOX, overview Nicotiana benthamiana (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
?
additional information Nb-9-LOX possesses both 9-lipoxygenase and 13-lipoxygenase, EC 1.13.11.12, specificity, with high predominance for the 9-LOX function Nicotiana benthamiana ?
-
?

Subunits

Subunits Comment Organism
? x * 97400, about, sequence calculation Nicotiana benthamiana

Synonyms

Synonyms Comment Organism
9-LOX
-
Nicotiana benthamiana
Nb-9-LOX
-
Nicotiana benthamiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Nicotiana benthamiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Nicotiana benthamiana

pI Value

Organism Comment pI Value Maximum pI Value
Nicotiana benthamiana sequence calculation
-
5.52

Expression

Organism Comment Expression
Nicotiana benthamiana the enzyme expression is highly induced by agroinfiltration of the plant leaves using a tobacco mosaic virus based vector system up