Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli | Arabidopsis thaliana |
heterologously expressed in Escherichia coli | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
A562G | the product of the wild-type enzyme is 98.8% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 1.2% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (99.4%). The mutant A562G forms (11S)-hydroperoxyanandamide and 15R-hydroperoxyanandamide in the ratio 3:2 | Arabidopsis thaliana |
A564G | the product of the wild-type enzyme is 99.1% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 0.9% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (71%), plus 16% (5S)-hydroperoxyanandamide. The mutant enzyme A564G forms two additional prominent products, 15-hydroperoxyanandamide (34%) and 9-hydroperoxyanandamide (19%) | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q06327 | - |
- |
Solanum lycopersicum | P38415 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified by nickel affinity chromatography using a N-terminal (His)6-tag | Arabidopsis thaliana |
partially purified by nickel affinity chromatography using a N-terminal (His)6-tag | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linoleate + O2 | - |
Arabidopsis thaliana | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | the product of the wild-type enzyme is 98.8% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 1.2% (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate. The product of mutant enzyme A562G is 68.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 31.1% (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | ? | |
linoleate + O2 | - |
Solanum lycopersicum | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | the product of the wild-type enzyme is 99.1% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 0.9% (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate. The product of mutant enzyme A564G is 59.9% (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and 40.1% (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | ? | |
additional information | from arachidonate the wild-type enzyme forms 11-hydroxyeicosatetraenoic acid and 5-hydroxyeicosatetraenoic acid in essentially equal amounts (38-39%), 11% 8-hydroxyeicosatetraenoic acid, 4% 12-hydroxyeicosatetraenoic acid, 3% 15-hydroxyeicosatetraenoic acid and 2% 9-hydroxyeicosatetraenoic acid. Wild-type enzyme converts anandamide mainly to (11S)-hydroperoxyanandamide (71%), plus 16% (5S)-hydroperoxyanandamide. The mutant enzyme A564G forms two additional prominent products, 15-hydroperoxyanandamide (34%) and 9-hydroperoxyanandamide (19%). No activity detected with 1-palmitoyl-2-linoleoylphosphatidylcholine. A model is tested that predicts a relationship between substrate binding orientation and product stereochemistry | Solanum lycopersicum | ? | - |
? | |
additional information | from arachidonate the wild-type enzyme forms 47% 11-hydroxyeicosatetraenoic acid, 23% 5-hydroxyeicosatetraenoic acid, 11% 9-hydroxyeicosatetraenoic acid, 9% 12-hydroxyeicosatetraenoic acid, 4.5% 8-hydroxyeicosatetraenoic acid and 4.5% 15-hydroxyeicosatetraenoic acid. Wild-type enzyme converts anandamide mainly to 11S-hydroperoxyanandamide (99.4%). The mutant A562G forms (11S)-hydroperoxyanandamide and (15R)-hydroperoxyanandamide in the ratio 3:2. No activity detected with 1-palmitoyl-2-linoleoylphosphatidylcholine. A model is tested that predicts a relationship between substrate binding orientation and product stereochemistry | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
9-LOX | - |
Arabidopsis thaliana |
9-LOX | - |
Solanum lycopersicum |
AtLOX1 | - |
Arabidopsis thaliana |
linoleate 9S-lipoxygenase | - |
Solanum lycopersicum |
LoxA | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
310 | - |
linoleate | - |
Arabidopsis thaliana | |
390 | - |
linoleate | - |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Arabidopsis thaliana |
6 | - |
- |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | pH 8: 30% of optimal activity, pH 9: about 15% of maximal activity | Solanum lycopersicum |