Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sphingomonas paucimobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%) | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0669 | - |
gallate | in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30°C | Sphingomonas paucimobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | DesB requires Fe2+ for activity | Sphingomonas paucimobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Sphingomonas paucimobilis |
46843 | - |
2 * 46843, calculated from amino acid sequence | Sphingomonas paucimobilis |
86000 | - |
gel filtration | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | Q5NTE5 | - |
- |
Sphingomonas paucimobilis SYK-6 | Q5NTE5 | - |
- |
Purification (Comment) | Organism |
---|---|
POROS polyethyleneimine column chromatography | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gallate + O2 | - |
Sphingomonas paucimobilis | 4-oxalomesaconate | - |
? | |
gallate + O2 | - |
Sphingomonas paucimobilis SYK-6 | 4-oxalomesaconate | - |
? | |
additional information | no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate | Sphingomonas paucimobilis | ? | - |
? | |
additional information | no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate | Sphingomonas paucimobilis SYK-6 | ? | - |
? | |
pyrogallol + O2 | - |
Sphingomonas paucimobilis | ? | - |
? | |
pyrogallol + O2 | - |
Sphingomonas paucimobilis SYK-6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Sphingomonas paucimobilis |
homodimer | 2 * 46843, calculated from amino acid sequence | Sphingomonas paucimobilis |
Synonyms | Comment | Organism |
---|---|---|
DesB | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sphingomonas paucimobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | - |
Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Sphingomonas paucimobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Sphingomonas paucimobilis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation | Sphingomonas paucimobilis |