General Stability | Organism |
---|---|
enzyme in crude extract is stable in Na-K phosphate buffer, pH 7.5, after 1 week of storage (2.4 mg of protein per ml) at 4°C, 100% of the original activity is recovered | Sphingobium xenophagum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
1,2-dihydroxynaphthalene | - |
Sphingobium xenophagum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for maximal activity | Sphingobium xenophagum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
8 * 33000, SDS-PAGE | Sphingobium xenophagum |
290000 | - |
gel filtration | Sphingobium xenophagum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxynaphthalene + O2 | Sphingobium xenophagum | induced in presence of naphthalene-2-sulfonic acid | 2-hydroxychromene-2-carboxylate | - |
? | |
1,2-dihydroxynaphthalene + O2 | Sphingobium xenophagum BN6 | induced in presence of naphthalene-2-sulfonic acid | 2-hydroxychromene-2-carboxylate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated | Sphingobium xenophagum |
Ethanol | storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated | Sphingobium xenophagum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium xenophagum | - |
BN6 | - |
Sphingobium xenophagum BN6 | - |
BN6 | - |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium xenophagum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83.36 | - |
- |
Sphingobium xenophagum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,5-trihydroxynaphthalene + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
1,2,6-trihydroxynaphthalene + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
1,2,7-trihydroxynaphthalene + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
1,2-dihydroxynaphthalene + O2 | induced in presence of naphthalene-2-sulfonic acid | Sphingobium xenophagum | 2-hydroxychromene-2-carboxylate | - |
? | |
1,2-dihydroxynaphthalene + O2 | induced in presence of naphthalene-2-sulfonic acid | Sphingobium xenophagum BN6 | 2-hydroxychromene-2-carboxylate | - |
? | |
1,2-dihydroxynaphthalene + O2 | - |
Sphingobium xenophagum | 2-hydroxy-2H-chromene-2-carboxylate | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Sphingobium xenophagum BN6 | ? | - |
? | |
3,4-dihydroxybiphenyl + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
3-methylcatechol + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
3-methylcatechol + O2 | - |
Sphingobium xenophagum BN6 | ? | - |
? | |
4-methylcatechol + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
4-methylcatechol + O2 | - |
Sphingobium xenophagum BN6 | ? | - |
? | |
catechol + O2 | - |
Sphingobium xenophagum | ? | - |
? | |
catechol + O2 | - |
Sphingobium xenophagum BN6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 33000, SDS-PAGE | Sphingobium xenophagum |
Synonyms | Comment | Organism |
---|---|---|
1,2-DHN dioxygenase | - |
Sphingobium xenophagum |
DHNDO | - |
Sphingobium xenophagum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is more stable at slightly alkaline pH values and better preserved in Na-K phosphate buffer than in Tris-HCI or glycine-NaOH | Sphingobium xenophagum |