Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | required for activity | Sulfobacillus acidophilus |
Cloned (Comment) | Organism |
---|---|
gene sor, DNA and amino acid sequence determination, comparison, and analysis, phylogenetic tree, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B21 in inclusion bodies | Sulfobacillus acidophilus |
Protein Variants | Comment | Organism |
---|---|---|
C101S | site-directed mutagenesis, 10% reamining activity compared to the wild-type enzyme | Sulfobacillus acidophilus |
C104S | site-directed mutagenesis, 47% reamining activity compared to the wild-type enzyme | Sulfobacillus acidophilus |
C31S | site-directed mutagenesis, inactive mutant | Sulfobacillus acidophilus |
E114A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Sulfobacillus acidophilus |
H86A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Sulfobacillus acidophilus |
H90A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Sulfobacillus acidophilus |
additional information | mutations of putative SOR active site residues, C31, C101, C104, H86 and H90, and E114: replacement of any cysteine residues reduced SORactivity by 53-100%, while the mutants of H86A, H90A and E114A lost their enzyme activities largely, only remaining 20%, 19% and 32% activity of the wild type SOR respectively | Sulfobacillus acidophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Sulfobacillus acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 sulfur + 4 H2O + O2 | Sulfobacillus acidophilus | - |
2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | Sulfobacillus acidophilus TPY | - |
2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfobacillus acidophilus | - |
isolated from a hydrothermal vent in the Pacific Ocean, gene sor | - |
Sulfobacillus acidophilus TPY | - |
isolated from a hydrothermal vent in the Pacific Ocean, gene sor | - |
Purification (Comment) | Organism |
---|---|
solubilized recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies by nickel affinity chromatography, and refolded by a four-step dialysis | Sulfobacillus acidophilus |
Renatured (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies, purified by nickel affinity chromatography, are refolded by a four-step dialysis using 20 mM Tris/HCl, 150 mM NaCl, pH 8.14, containing 2 mM reduced glutathione, 0.02 mM oxidized glutathione, 5% glycerol, 0.005% Tween 20, and 0.1 mM ferric citrate with 6 M urea in the first step, 3 M urea in the second step, and 1 M urea in the third step | Sulfobacillus acidophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
wild-type enzyme in absence of GSH, pH 8.0, 80°C | Sulfobacillus acidophilus |
1.75 | - |
mutant C101S, pH 8.0, 80°C | Sulfobacillus acidophilus |
3.28 | - |
mutant H90A, pH 8.0, 80°C | Sulfobacillus acidophilus |
3.47 | - |
mutant H86A, pH 8.0, 80°C | Sulfobacillus acidophilus |
5.46 | - |
mutant E114A, pH 8.0, 80°C | Sulfobacillus acidophilus |
8.09 | - |
mutant C104S, pH 8.0, 80°C | Sulfobacillus acidophilus |
17.17 | - |
wild-type enzyme in presence of GSH, pH 8.0, 80°C | Sulfobacillus acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 sulfur + 4 H2O + O2 | - |
Sulfobacillus acidophilus | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | - |
Sulfobacillus acidophilus TPY | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Sulfobacillus acidophilus |
Synonyms | Comment | Organism |
---|---|---|
SOR | - |
Sulfobacillus acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Sulfobacillus acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sulfobacillus acidophilus |