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Literature summary for 1.13.11.55 extracted from
- Site-directed mutagenesis reveals new and essential elements for iron-coordination of the sulfur oxygenase reductase from the acidothermophilic Acidianus tengchongensis (2009), Chin. Sci. Bull., 54, 652-657.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild type and mutant enzyme in Escherichia coli | Acidianus tengchongensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C101S | 1.95 mol iron content per mol subunit, cysteine residue is essential for activity | Acidianus tengchongensis |
| C104S | 2.4 mol iron content per mol subunit, cysteine residue is essential for activity | Acidianus tengchongensis |
| C31S | 1.86 mol iron content per mol subunit, cysteine residue is essential for activity | Acidianus tengchongensis |
| E129A | site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0.81 mol iron content per mol subunit | Acidianus tengchongensis |
| H86F | site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0 mol iron content per mol subunit | Acidianus tengchongensis |
| H90F | site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0.6 mol iron content per mol subunit | Acidianus tengchongensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | Fe2+-specific chelator, strong inhibition | Acidianus tengchongensis |  |
| 4,5-dihydroxy-meta-benzenedisulfonic acid | tiron, Fe3+-specific chelator, strong inhibition | Acidianus tengchongensis | |
| 8-hydroxyquinoline | Fe3+-specific chelator, strong inhibition | Acidianus tengchongensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | essential for activity, recombinant SOR had a molar ratio of 1.86:1 (iron to subunit of SOR), excesses of ferric and ferrous ions have inhibitory effect on SOR activity | Acidianus tengchongensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Acidianus tengchongensis | SOR catalyzes simultaneously oxidation and reduction of elementar sulfur to produce sulfite, thiosulfate and sulfide, in the presence of molecule oxygen | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidianus tengchongensis | Q977W3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by gel filtration | Acidianus tengchongensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0% activity with 0.2 mM 2.2'-dipyridyl compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
0.3% activity with 1 mM Fe3+ compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
17.8% activity with 1.0 mM 8-hydroxyquinoline compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
199.4% activity with 10 mM 2.2'-dipyridyl after ultrafiltration compared to activity without any treatment, the SOR activity recovers after removal (washing/untrafiltration) of the excessive iron | Acidianus tengchongensis |
| additional information | - |
21.1% activity with 0.04 mM 2.2'-dipyridyl compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
239.7% activity with 10 mM 4,5-dihydroxy-meta-benzenedisulfonic acid after ultrafiltration compared to activity without any treatment, the SOR activity recovers after removal (washing and ultrafiltration) of the excessive iron | Acidianus tengchongensis |
| additional information | - |
31.5% activity with 1 mM Fe2+ compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
44% activity with 0.1 mM 8-hydroxyquinoline compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
52.8% activity with 1.0 mM 4,5-dihydroxy-meta-benzenedisulfonic acid compared to activity without any treatment | Acidianus tengchongensis |
| additional information | - |
55% activity with 0.1 mM 4,5-dihydroxy-meta-benzenedisulfonic acid compared to activity without any treatment | Acidianus tengchongensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | SOR catalyzes simultaneously oxidation and reduction of elementar sulfur to produce sulfite, thiosulfate and sulfide, in the presence of molecule oxygen | Acidianus tengchongensis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOR | - |
Acidianus tengchongensis |
| sulfur oxygenase reductase | - |
Acidianus tengchongensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Acidianus tengchongensis |
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