General Stability | Organism |
---|---|
loses more than 80% of activity after three freezing and thawing steps | Acidianus ambivalens |
partially inactivated by freezing at -80°C | Acidianus ambivalens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-iodoacetic acid | - |
Acidianus ambivalens | |
4-chloromercuribenzoic acid | - |
Acidianus ambivalens | |
FAD | - |
Acidianus ambivalens | |
Fe2+ | - |
Acidianus ambivalens | |
Fe3+ | - |
Acidianus ambivalens | |
additional information | the enmzyme is not affected by CN-, N3-, or reduced glutathione | Acidianus ambivalens | |
N-ethylmaleimide | - |
Acidianus ambivalens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Acidianus ambivalens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Acidianus ambivalens |
550000 | - |
gel filtration | Acidianus ambivalens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus ambivalens | P29082 | - |
- |
Acidianus ambivalens DSM 3772 | P29082 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidianus ambivalens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:aerobically-grown cell | not detectable in anaerobically grown cells | Acidianus ambivalens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.6 | - |
pH 7.4, 85°C | Acidianus ambivalens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 sulfur + 4 H2O + O2 | in the presence of oxygen but not under a hydrogen atmosphere, the enzyme simultaneously produces sulfite, thiosulfate, and hydrogen sulfide from sulfur. Nonenzymatic control experiments show that thiosulfate is produced mainly in a chemical reaction between sulfite and sulfur. The ratio of sulfite to hydrogen sulfide production is 5:4 in the presence of zinc ions | Acidianus ambivalens | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? | |
4 sulfur + 4 H2O + O2 | in the presence of oxygen but not under a hydrogen atmosphere, the enzyme simultaneously produces sulfite, thiosulfate, and hydrogen sulfide from sulfur. Nonenzymatic control experiments show that thiosulfate is produced mainly in a chemical reaction between sulfite and sulfur. The ratio of sulfite to hydrogen sulfide production is 5:4 in the presence of zinc ions | Acidianus ambivalens DSM 3772 | 2 hydrogen sulfide + 2 bisulfite + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Acidianus ambivalens |
Synonyms | Comment | Organism |
---|---|---|
SOR | - |
Acidianus ambivalens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Acidianus ambivalens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 100 | 75°C: about 40% of maximal activity | Acidianus ambivalens |