Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella oxytoca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
O2 | - |
Klebsiella oxytoca | |
210 | - |
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one | - |
Klebsiella oxytoca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | apoenzyme is catalytically inactive. Addition of Fe2+ yields activity. Production of the enzyme in intact Escherichia coli depends on the availability of the Fe2+. Enzyme contains 0.9 Fe2+ per enzyme molecule | Klebsiella oxytoca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20240 | - |
mass spectrometry | Klebsiella oxytoca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | Klebsiella oxytoca | enzyme of the methionine salvage pathway | 4-(methylthio)-2-oxobutanoate + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | Q9ZFE7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Klebsiella oxytoca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | enzyme of the methionine salvage pathway | Klebsiella oxytoca | 4-(methylthio)-2-oxobutanoate + formate | - |
? | |
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | Fe2+ bound to the enzyme protein. If Ni2+ or Co2+ is bound instead of Fe+, the reaction catalzed by EC 1.13.11.53 occurs instead | Klebsiella oxytoca | 4-(methylthio)-2-oxobutanoate + formate | - |
? |