Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Mus musculus | |
methylene blue | - |
Mus musculus |
Cloned (Comment) | Organism |
---|---|
isozyme IDO2 is expressed in KRX cells, isozyme IDO1 is expressed in Escherichia coli Rosetta cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-methyl-D-tryptophan | poor non-competitive inhibitor of both IDO1 and IDO2 activity | Mus musculus | |
1-methyl-L-tryptophan | poor competitive inhibitor of isozyme IDO1 | Mus musculus | |
nitric oxide | nitric oxide can potentially inhibit isozyme IDO2 activity | Mus musculus | |
norharman | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
L-tryptophan | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
0.029 | - |
L-tryptophan | recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
0.53 | - |
L-tryptophan | recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
12 | - |
L-tryptophan | recombinant isozyme IDO2, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
isozymes IDO1 and IDO2, SDS-PAGE | Mus musculus |
45200 | - |
isozyme IDO2, calculated from amino acid sequence | Mus musculus |
45300 | - |
isozyme IDO1, calculated from amino acid sequence | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Mus musculus | - |
N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | Mus musculus | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-L-tryptophan + O2 | - |
Mus musculus | N-formyl-5-hydroxy-L-kynurenine | - |
? | |
D-tryptophan + O2 | - |
Mus musculus | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Mus musculus | N-formyl-L-kynurenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, isozymes IDO1 and IDO2, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
IDO1 | isozyme | Mus musculus |
IDO2 | isozyme | Mus musculus |
indoleamine 2,3-dioxygenase-1 | - |
Mus musculus |
indoleamine 2,3-dioxygenase-2 | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | 60 | the melting temperature of the denaturation process for recombinant isozyme IDO1 is 60°C, with a possible second intermediate at 48°C, recombinant isozyme IDO2 denatures in a single step, with a melting temperature at 48°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | isozyme IDO1 | Mus musculus |
7.4 | 7.5 | isozyme IDO2 | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | recombinant isozymes IDO2 and IDO1 are ferric (Fe3+) type heme proteins | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
1-methyl-L-tryptophan | recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
0.105 | - |
1-methyl-L-tryptophan | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
0.44 | - |
1-methyl-L-tryptophan | recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
0.69 | - |
norharman | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
1.08 | - |
norharman | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
1.73 | - |
norharman | recombinant isozyme IDO1, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
1.96 | - |
norharman | recombinant isozyme IDO2, in the presence of 50 nM recombinant human cytochrome b5 and 50 nM NADPH cytochrome P450 reductase, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
2.75 | - |
1-methyl-L-tryptophan | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus | |
11.3 | - |
1-methyl-D-tryptophan | recombinant isozyme IDO1, in the presence of 0.01 mM methylene blue, in 100 mM phosphate buffer, pH 7.4, at 37°C | Mus musculus |