Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tryptophan | substrate inhibition at concentrations above 0.04 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-tryptophan | pH 7.5, 37 °C, 5 min incubation time | Mus musculus | |
0.062 | - |
D-tryptophan | pH 7.5, 37 °C, 40 min incubation time | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Mus musculus | - |
N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | Mus musculus | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogenization, ammonium sulfate precipitation, gel filtration with Sephacryl S200, Bio-gel HT column, stored at -75°C | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epididymis | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-75°C | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | - |
Mus musculus | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Mus musculus | N-formyl-L-kynurenine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | 4-5-fold higher activity than acitivity with methylene blue as the electron donor | Mus musculus | |
tetrahydrobiopterin | - |
Mus musculus |