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Literature summary for 1.13.11.52 extracted from

  • Littlejohn, T.K.; Takikawa, O.; Truscott, R.J.W.; Walker, M.J.
    Asp274 and His346 are essential for heme binding and catalytic function of human indolamine 2,3-dioxygenase (2003), J. Biol. Chem., 278, 29525-29531.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
gamma-interferon
-
Homo sapiens

Application

Application Comment Organism
medicine enzyme catalyzes the first and rate-limiting step in kynurenine pathway Homo sapiens

Protein Variants

Protein Variants Comment Organism
D274A mutant without enzyme activity, may be distal ligand or essential in maintaining the conformation of the heme pocket Homo sapiens
H16A does not act as proximal ligand Homo sapiens
H303A does not act as proximal ligand Homo sapiens
H346A mutant without enzyme activity, His346 is essential for heme binding Homo sapiens
K352A mutant with diminished heme binding ability Homo sapiens
V109A mutant maintained heme binding ability Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
D-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0024
-
D-tryptophan H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0044
-
D-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.005
-
D-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0053
-
D-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0056
-
L-tryptophan H16A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0089
-
L-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0094
-
L-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.02
-
L-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.0201
-
L-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.1
-
5-hydroxy-L-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.1
-
5-hydroxy-L-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.14
-
5-hydroxy-L-tryptophan H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.17
-
5-hydroxy-L-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
0.4
-
5-hydroxy-L-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-tryptophan + O2
-
Homo sapiens N-formyl-5-hydroxy-L-kynurenine
-
?
D-tryptophan + O2
-
Homo sapiens N-formyl-D-kynurenine
-
?
L-tryptophan + O2
-
Homo sapiens N-formyl-L-kynurenine
-
?

Synonyms

Synonyms Comment Organism
IDO
-
Homo sapiens