Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | active site nonheme monoiron(II) center, facially ligated by three histidine residues, overview. Spectral analysis of Dke1 FeII-alpha-keto acid complexes with 4-hydroxyphneylpyruvate, overview | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acinetobacter johnsonii | acetylacetone dioxygenase catalyzes the dioxygen-dependent degradation of beta-dicarbonyl compounds | ? | - |
? | |
pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + methylglyoxal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acetylacetone dioxygenase catalyzes the dioxygen-dependent degradation of beta-dicarbonyl compounds | Acinetobacter johnsonii | ? | - |
? | |
additional information | Dke1 also performs the atypical cleavage of the alpha-keto acid, 4-hydroxyphenylpyruvate, to form 4-hydroxybenzaldehyde as product instead of the homogentisate product found for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase, EC 1.13.11.27, analysis of the bonding of the alpha-keto acid, 4-hydroxyphenylpyruvate, to ferrous Dke1 using anaerobic Dke1, added ferrous ammonium sulfate, and 4-hydroxyphenylpyruvate at pH 7.0, overview | Acinetobacter johnsonii | ? | - |
? | |
pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + methylglyoxal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetylacetone dioxygenase | - |
Acinetobacter johnsonii |
diketone cleaving dioxygenase | - |
Acinetobacter johnsonii |
Dke1 | - |
Acinetobacter johnsonii |
General Information | Comment | Organism |
---|---|---|
additional information | Dke1 also performs the atypical cleavage of the alpha-keto acid, 4-hydroxyphenylpyruvate, to form 4-hydroxybenzaldehyde as product instead of the homogentisate product found for 4-hydroxyphenylpyruvate with 4-hydroxyphenylpyruvate dioxygenase, EC 1.13.11.27, analysis of the bonding of the alpha-keto acid, 4-hydroxyphenylpyruvate, to ferrous Dke1 using anaerobic Dke1, added ferrous ammonium sulfate, and 4-hydroxyphenylpyruvate at pH 7.0, overview. active site was modeling, histidine residues are truncated to methyl imidazole for the model, and constraints imposed by the protein backbone are simulated by fixing the relative positions of the beta-carbons of the backbone. The coordination of the active site is completed with either alpha keto (monoanion) or enolate (dianion) bidentate coordinated 4-hydroxyphenylpyruvate ligand or a monoanionic acetylacetone ligand | Acinetobacter johnsonii |