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Literature summary for 1.13.11.5 extracted from
- Purification of the homogentisic acid oxidase from mammalian liver (1995), Int. J. Biochem. Cell Biol., 27, 1357-1363.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Mus musculus |
| medicine | inactivation of enzyme in kidney and liver causes the basic defect of alkaptonuria | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | human gene for alkaptonuria is mapped to chromosome 3q2 | Homo sapiens |
| additional information | alkaptonuric mouse lacks enzyme activity and have recessive mutation aku, mapped to chromosome 16, inbred strain C57BLG/J is heterozygous for the aku mutation | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 0.001 mM, 56% inhibition | Oryctolagus cuniculus |  |
| HgCl2 | 20 mM, complete inhibition | Oryctolagus cuniculus | |
| NaCl | 0.001 mM, 63% inhibition | Oryctolagus cuniculus | |
| p-hydroxyphenylacetic acid | 80 mM, complete inhibition, substrate analogue | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
homogentisate | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Mus musculus |
| 450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Homo sapiens |
| 450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | Mus musculus | - |
4-maleylacetoacetate | - |
? | |
| homogentisate + O2 | Homo sapiens | enzyme catalyzes an intermediate step in the catabolism of tyrosine and phenylalanine | 4-maleylacetoacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
normal and alkaptonuric mice, which completely lack HTO | - |
| Oryctolagus cuniculus | - |
male rabbits | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| inactivation by air probably causes degradation of enzyme in two proteins | Oryctolagus cuniculus |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | enzyme is not glycosylated | Oryctolagus cuniculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
- |
Homo sapiens |
- |
Bos taurus |
| 54.1fold purification | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | liver from a 20 week old male embryo | Homo sapiens | - |
| liver | - |
Mus musculus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | embryonal liver | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
| additional information | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | - |
Mus musculus | 4-maleylacetoacetate | - |
? | |
| homogentisate + O2 | - |
Homo sapiens | 4-maleylacetoacetate | - |
? | |
| homogentisate + O2 | - |
Oryctolagus cuniculus | 4-maleylacetoacetate | - |
? | |
| homogentisate + O2 | enzyme catalyzes an intermediate step in the catabolism of tyrosine and phenylalanine | Homo sapiens | 4-maleylacetoacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 450000-480000, SDS-PAGE | Mus musculus |
| monomer | 1 * 450000-480000, SDS-PAGE | Homo sapiens |
| monomer | 1 * 450000-480000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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