Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on the presence of ascorbate, presumably to maintain iron in the reduced form | Aspergillus nidulans | |
additional information | enzymes of phenylalanine catabolism including HGO are strongly induced by the presence of phenylalanine or phenylacetate | Aspergillus nidulans |
Application | Comment | Organism |
---|---|---|
diagnostics | crude enzyme preparations can be used for a spectrophotometric method for routine, sensitive determination of homogentisate in human urine | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | strain carrying an homogentisate dioxygenase gene disruption with full genotype biA1, methG1, deltahmgA without enzyme activity | Aspergillus nidulans |
General Stability | Organism |
---|---|
rather stable enzyme, can be repeatedly thawed and frozen without significant loss of activity | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-Dihydroxyphenylacetate | 1 mM, 48% inhibition, substrate analogue | Aspergillus nidulans | |
p-hydroxyphenylpyruvate | 1 mM, 68% inhibition, substrate analogue | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
homogentisate | - |
Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on the presence of Fe2+ | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | Aspergillus nidulans | one of the enzymes mediating phenylalanine catabolism | 4-maleylacetoacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
filamentous ascomycete | - |
Aspergillus nidulans | - |
strains biA1 and veA1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.75 | 1 | value in crude lysates | Aspergillus nidulans |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.0, crude extracts, 5 months, 25% loss of activity | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | highly specific for homogentisate | Aspergillus nidulans | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | one of the enzymes mediating phenylalanine catabolism | Aspergillus nidulans | 4-maleylacetoacetate | - |
? | |
additional information | not as substrates: phenylacetate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 4-hydroxyphenylacetate, phenylalanine, tyrosine, phenylpyruvate, gentisate | Aspergillus nidulans | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Aspergillus nidulans |