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Literature summary for 1.13.11.5 extracted from
- Purification and properties of homogentisate oxygenase from Pseudomonas fluorescens (1966), Biochim. Biophys. Acta, 118, 88-97.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required for maximal activity at pH 6 | Pseudomonas fluorescens |  |
| cysteine | activates | Pseudomonas fluorescens | |
| glutathione | required for maximal activity at pH 6 and 5.4 | Pseudomonas fluorescens | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
General Stability
| General Stability | Organism |
|---|---|
| unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pseudomonas fluorescens | |
| p-chloromercuribenzoate | competitive inhibition with respect to Fe2+ but not to homogentisate | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
homogentisate | at pH 6 | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Fe2+ is required as cofactor | Pseudomonas fluorescens | |
| additional information | other metallic ions, such as Fe3+, Co2+, Mn2+, Cu+ and Cu2+, cannot replace Fe2+ | Pseudomonas fluorescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 380000 | - |
ultracentrifugal analysis | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to O2, easily inactivated by aeration | Pseudomonas fluorescens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.25 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 6.0-7.2, in absence of Fe2+, several weeks, stable | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | specific for homogentisate | Pseudomonas fluorescens | 4-maleylacetoacetate | - |
? | |
| additional information | not as substrates: salicylic acid, catechol, 2,3-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxybenzoic acid, o-hydroxyphenylacetic acid, p-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid | Pseudomonas fluorescens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.2 | -20°C, stable for several weeks | Pseudomonas fluorescens |
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