Any feedback?
Literature summary for 1.13.11.5 extracted from
- Nonheme iron dioxygenase (1974), Mol. Mech. Oxygen Activ. (Hayaishi, O., ed.) Academic Press, New York, , 135-165.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required for maximal activity at pH 6 | Pseudomonas fluorescens |  |
| ascorbate | required for maximal activity at pH 7 | Bos taurus | |
| glutathione | required for maximal activity at pH 6 and 5.4 | Pseudomonas fluorescens | |
| glutathione | reduced glutathione is required for maximal activity at pH 7 | Bos taurus | |
| additional information | pH 7.0: a combination of ascorbate, reduced glutathione and ferrous iron is required for activation, pH 5.3: ferrous iron alone fully activates | Bos taurus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
General Stability
| General Stability | Organism |
|---|---|
| 10% acetone stabilizes | Bos taurus |
| unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Lewisite | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
| Methylmercuric bromide | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
| p-chloromercuribenzoate | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
| p-chloromercuribenzoate | competitive inhibition with respect to Fe2+ but not to homogentisate | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
homogentisate | at pH 6 | Pseudomonas fluorescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | ferrous mercaptans at the active center | Bos taurus | |
| Fe2+ | Fe2+ is required as cofactor | Pseudomonas fluorescens | |
| Fe2+ | ferrous ion is required for maximal activity at pH 7 and pH 5.3 | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 380000 | - |
ultracentrifugal analysis | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| Fe2+, organic mercurials and reducing agents protect the enzyme from irreversible aerobic oxidation | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| homogentisate + O2 | - |
Pseudomonas fluorescens | 4-maleylacetoacetate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
