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Literature summary for 1.13.11.49 extracted from

  • Streit, B.R.; Blanc, B.; Lukat-Rodgers, G.S.; Rodgers, K.R.; DuBois, J.L.
    How active-site protonation state influences the reactivity and ligation of the heme in chlorite dismutase (2010), J. Am. Chem. Soc., 132, 5711-5724.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the gene encoding mature Cld, secretion peptide removed and replaced with an N-terminal Met residue, is expressed in untagged form from the pET41a vector in Tuner(DE3) Escherichia coli cells Dechloromonas aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, and steady-state pH-rate profiles, overview Dechloromonas aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme protein, formation of heme-hydroxide complexes, overview Dechloromonas aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chloride + O2 Dechloromonas aromatica chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity chlorite
-
r

Organism

Organism UniProt Comment Textmining
Dechloromonas aromatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mature Cld from Tuner(DE3) Escherichia coli cells by anion exchange chromatography and gel filtration Dechloromonas aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chloride + O2 chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity Dechloromonas aromatica chlorite
-
r
chloride + O2 chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity, the protonation state of the heme environment influences dioxygen formation. The reaction proceeds via an acid-base transition with pKa of 8.7, spectroscopic properties, ligand binding affinities, and steady-state kinetics, unique structure-activity model, overview Dechloromonas aromatica chlorite
-
r

Synonyms

Synonyms Comment Organism
chlorite dismutase
-
Dechloromonas aromatica
CLD
-
Dechloromonas aromatica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Dechloromonas aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Dechloromonas aromatica

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 activity range, pH-rate profiles and pH-dependent ligand binding constants, active and inactive enzyme forms dependent on the protonation status, steady-state pH-rate profiles, overview Dechloromonas aromatica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 the activity decreases dramatically below pH 5 and above pH 10, loss of activity is irreversible and accompanied by dramatic and rapid changes in the heme UV/vis chromophore, overview Dechloromonas aromatica

Cofactor

Cofactor Comment Organism Structure
heme
-
Dechloromonas aromatica

General Information

General Information Comment Organism
additional information active and inactive enzyme forms dependent on the protonation status, overview Dechloromonas aromatica