Cloned (Comment) | Organism |
---|---|
the gene encoding mature Cld, secretion peptide removed and replaced with an N-terminal Met residue, is expressed in untagged form from the pET41a vector in Tuner(DE3) Escherichia coli cells | Dechloromonas aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, and steady-state pH-rate profiles, overview | Dechloromonas aromatica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme protein, formation of heme-hydroxide complexes, overview | Dechloromonas aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloride + O2 | Dechloromonas aromatica | chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity | chlorite | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dechloromonas aromatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mature Cld from Tuner(DE3) Escherichia coli cells by anion exchange chromatography and gel filtration | Dechloromonas aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloride + O2 | chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity | Dechloromonas aromatica | chlorite | - |
r | |
chloride + O2 | chlorite dismutase catalyzes O2 release from chlorite with exquisite efficiency and specificity, the protonation state of the heme environment influences dioxygen formation. The reaction proceeds via an acid-base transition with pKa of 8.7, spectroscopic properties, ligand binding affinities, and steady-state kinetics, unique structure-activity model, overview | Dechloromonas aromatica | chlorite | - |
r |
Synonyms | Comment | Organism |
---|---|---|
chlorite dismutase | - |
Dechloromonas aromatica |
CLD | - |
Dechloromonas aromatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Dechloromonas aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Dechloromonas aromatica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | activity range, pH-rate profiles and pH-dependent ligand binding constants, active and inactive enzyme forms dependent on the protonation status, steady-state pH-rate profiles, overview | Dechloromonas aromatica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | the activity decreases dramatically below pH 5 and above pH 10, loss of activity is irreversible and accompanied by dramatic and rapid changes in the heme UV/vis chromophore, overview | Dechloromonas aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Dechloromonas aromatica |
General Information | Comment | Organism |
---|---|---|
additional information | active and inactive enzyme forms dependent on the protonation status, overview | Dechloromonas aromatica |