Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | at 70 microM 44% inhibition, at 700 microM 89% inhibition | Stutzerimonas chloritidismutans | |
cyanide | at 70 microM 83% inhibition, at 700 microM 95% inhibition | Stutzerimonas chloritidismutans | |
EDTA | at 70 microM 13% inhibition, at 700 microM 30% inhibition | Stutzerimonas chloritidismutans | |
H2O2 | at 70 microM 6% inhibition, at 700 microM 37% inhibition | Stutzerimonas chloritidismutans | |
additional information | excess chlorite inactivates and bleaches the enzyme, 80% activity loss with 56 mM chlorite, 90% after second application of 56 mM chlorite; no inhibition by hydroxylamine or 3-amino-1,2,3-triazole | Stutzerimonas chloritidismutans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Stutzerimonas chloritidismutans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | activity increases with increasing salt concentration up to 2 M | Stutzerimonas chloritidismutans | |
additional information | kosmotropic salts such as sulfate and phosphate stabilize protein structures by water-structuring effects of the ions, chaotropic salts such as nitrate and chlorate reduce the activity, no effect of NaCl, NH4Cl, and NaHCO3 (up to 1.5 M) on enzyme activity | Stutzerimonas chloritidismutans | |
Na2SO4 | activity increases with increasing salt concentration up to 2 M | Stutzerimonas chloritidismutans | |
NaClO3 | decreasing activity with increasing salt concentration | Stutzerimonas chloritidismutans | |
NaNO3 | decreasing activity with increasing salt concentration | Stutzerimonas chloritidismutans | |
potassium phosphate | activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt | Stutzerimonas chloritidismutans | |
Sodium phosphate | activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt | Stutzerimonas chloritidismutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) | Stutzerimonas chloritidismutans |
110000 | - |
SDS-PAGE, native enzyme with Superdex 200 column with 50 mM potassium phosphate buffer, pH 7.0 and 100 mM NaCl | Stutzerimonas chloritidismutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | Stutzerimonas chloritidismutans | - |
chloride + O2 | water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) | ? | |
chlorite | Stutzerimonas chloritidismutans AW-1 / DSM 13592 | - |
chloride + O2 | water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stutzerimonas chloritidismutans | - |
- |
- |
Stutzerimonas chloritidismutans AW-1 / DSM 13592 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell pellet suspended in 0.05 M Tris-HCl buffer, pH 9.5 and 0.05 EDTA for 30 min at room temperature, centrifuged, supernatant ultracentrifuged, red supernatant containing periplasmic fraction separated from pellet, diluted with 10 mM Tris-HCl buffer, pH 7.2, purification with ÄKTAfplc, diluted supernatant loaded onto hydroxyapatite column with 10 mM Tris-HCl, pH 7.2, enzyme elutes at the start of a linear gradient of 10 mM Tris-Hcl, pH 7.2 to 450 mM potassium phosphate, pH 7.2, storage at -20°C | Stutzerimonas chloritidismutans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the formation of O2 is influenced by the concentrations of enzyme and chlorite in 1 M phosphate buffer, pH 6.0, at 25°C, undiluted chlorite dismutase (0.64 mg/l) produces 98 microM O2 with 100 microM ClO2-, 205 microM O2 with 200 microM ClO2-, 300 microM O2 with 300 microM ClO2-, 10 times diluted chlorite dismutase (0.064 mg/l) produces 34 microM O2 with 100 microM ClO2-, 69 microM O2 with 200 microM ClO2-, 96 microM O2 with 300 microM ClO2- | Stutzerimonas chloritidismutans |
443 | - |
Vmax, 100 mM sodium phosphate buffer, pH 6.0, 25°C, 0.2 mM chlorite | Stutzerimonas chloritidismutans |
4650 | - |
Vmax increase with 1 M phosphate, pH 6.0, 25°C | Stutzerimonas chloritidismutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | - |
Stutzerimonas chloritidismutans | chloride + O2 | water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) | ? | |
chlorite | - |
Stutzerimonas chloritidismutans AW-1 / DSM 13592 | chloride + O2 | water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) | ? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) | Stutzerimonas chloritidismutans |
Synonyms | Comment | Organism |
---|---|---|
chlorite dismutase | - |
Stutzerimonas chloritidismutans |
CLD | - |
Stutzerimonas chloritidismutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
100 mM sodium phosphate buffer, pH 7.2 | Stutzerimonas chloritidismutans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | 100 mM sodium phosphate buffer, pH 7.2 | Stutzerimonas chloritidismutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
sodium phosphate buffer, 25°C | Stutzerimonas chloritidismutans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | sodium phosphate buffer, 25°C | Stutzerimonas chloritidismutans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2700 | - |
Chlorite | 100 mM sodium phosphate buffer, pH 6.0, 25°C | Stutzerimonas chloritidismutans |