Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Arthrobacter sp. | |
Ca2+ | weak | Arthrobacter sp. | |
Co2+ | weak | Arthrobacter sp. | |
Cu2+ | strong | Arthrobacter sp. | |
Cu2+ | - |
Pseudomonas putida | |
ethylxanthate | - |
Arthrobacter sp. | |
Fe2+ | strong | Arthrobacter sp. | |
H2O2 | - |
Arthrobacter sp. | |
H2O2 | - |
Pseudomonas putida | |
iodoacetamide | - |
Arthrobacter sp. | |
Mg2+ | weak | Arthrobacter sp. | |
Mn2+ | weak | Arthrobacter sp. | |
NEM | - |
Arthrobacter sp. | |
Ni2+ | strong | Arthrobacter sp. | |
p-hydroxymercuribenzoate | - |
Arthrobacter sp. | |
Sodium azide | - |
Arthrobacter sp. | |
Sodium dithionite | - |
Arthrobacter sp. | |
Zn2+ | strong | Arthrobacter sp. | |
Zn2+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
1H-3-Hydroxy-4-oxoquinoline | - |
Pseudomonas putida | |
0.03 | - |
1H-3-Hydroxy-4-oxoquinaldine | - |
Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | contains 0.048 mol of Cu per mol of enzyme | Pseudomonas putida | |
additional information | addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity | Pseudomonas putida | |
additional information | addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity | Arthrobacter sp. | |
Nickel | contains 0.038 mol of Ni per mol of enzyme | Pseudomonas putida | |
Nickel | contains 0.02 mol of Ni per mol of enzyme | Arthrobacter sp. | |
Zinc | contains 0.045 mol of zinc per mol of enzyme | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Pseudomonas putida |
32000 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
strain R³61a | - |
Arthrobacter sp. Ru61a | - |
strain R³61a | - |
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 33/1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
- |
Arthrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:quinaldine-grown cell | - |
Arthrobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.1 | - |
- |
Pseudomonas putida |
70.7 | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1H-3-Hydroxy-4-oxoquinaldine + O2 | - |
Arthrobacter sp. | N-Acetylanthranilate + CO | - |
? | |
1H-3-Hydroxy-4-oxoquinaldine + O2 | - |
Arthrobacter sp. Ru61a | N-Acetylanthranilate + CO | - |
? | |
1H-3-Hydroxy-4-oxoquinoline + O2 | - |
Pseudomonas putida | N-Formylanthranilate + CO | - |
? | |
1H-3-Hydroxy-4-oxoquinoline + O2 | at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine | Arthrobacter sp. | N-Formylanthranilate + CO | - |
? | |
1H-3-Hydroxy-4-oxoquinoline + O2 | - |
Pseudomonas putida 33/1 | N-Formylanthranilate + CO | - |
? | |
1H-3-Hydroxy-4-oxoquinoline + O2 | at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine | Arthrobacter sp. Ru61a | N-Formylanthranilate + CO | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 32000, SDS-PAGE | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas putida |
30 | - |
- |
Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
labile above | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
8 | - |
- |
Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no spectral evidence for the presence of a chromophoric cofactor | Pseudomonas putida | |
additional information | no spectral evidence for the presence of a chromophoric cofactor | Arthrobacter sp. |