Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Gaeumannomyces graminis |
Protein Variants | Comment | Organism |
---|---|---|
G316A | site-directed mutagenesis, the mutant changes the the position of lipoxygenation toward the carboxyl group of 20:2n-6 and 20:3n-3 and prevents the bis-allylic hydroperoxide of 20:3n-3 but not of 20:2n-6to interact with the catalytic metall | Gaeumannomyces graminis |
N466L | site-directed mutagenesis, the mutation hardly affects the bis-allylic hydroperoxide rearrangement | Gaeumannomyces graminis |
S469A | site-directed mutagenesis, the mutation hardly affects the bis-allylic hydroperoxide rearrangement, the mutant shows the same substrate profile as the wild-type enzyme | Gaeumannomyces graminis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
linoleate | pH 7.5, 18:3n-3 substrate | Gaeumannomyces graminis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Gaeumannomyces graminis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity | Gaeumannomyces graminis | |
additional information | no activity with Fe3+ | Gaeumannomyces graminis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaeumannomyces graminis | - |
take-all fungus | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
linoleate + O2 = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate | reaction mechanism, tail-first model with rearrangement at C13 at the end of lipoxygenation, influenced by double bond configuration and the chain length of fatty acids, overview | Gaeumannomyces graminis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(11Z,14Z)-eicosadienoic acid + O2 | - |
Gaeumannomyces graminis | 15-hydroxyperoxy-(9Z,11E)-eicosadienoic acid | - |
? | |
(11Z,14Z,17Z)-eicosa-11,14,17-trienoic acid + O2 | via 11-hydroperoxyoctadecadienoic acid, 13-hydroperoxyoctadecadienoic acid and 15-hydroperoxyoctadecadienoic acid | Gaeumannomyces graminis | 15-hydroxyperoxy-11Z,13E,17Z-eicosatrienoic acid | - |
? | |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | oxidation at the n-3 position | Gaeumannomyces graminis | ? | - |
? | |
1-linoleyl-lyso-glycerophosphatidylcholine + O2 | via formation of the 13-hydroxyperoxy metabolite | Gaeumannomyces graminis | 11-hydroxyoctadecanoic acid + 13-hydroxyoctadecanoic acid | - |
? | |
11-hydroperoxyoctadecadienoic acid + O2 | - |
Gaeumannomyces graminis | 11,18-dihydroxy-12E,14Z,16E-eicosatrienoic acid | two diastereoisomers | ? | |
alpha-linolenic acid + O2 | - |
Gaeumannomyces graminis | (13R)-hydroperoxy octadecadienoic acid + 9-hydroperoxyoctadecadienoic acid | - |
? | |
linoleate + O2 | - |
Gaeumannomyces graminis | (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
linoleic acid + O2 | - |
Gaeumannomyces graminis | ? | - |
? | |
additional information | the enzyme catalyzes the rearrangement of bis-allylic S-hydroxyperoxides to allylic R-hydroperoxides, and the oxygenation of 18:2n-6 by suprafacial hydrogen abstraction at C11 and O2 insertion at the bis-allylic position C11 and, with double bond migation, at the allylic position C13, overview, 20:4n-6 is a poor substrate, substrate specificities of wild-type and mutant enzymes, overview | Gaeumannomyces graminis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
manganese lipoxygenase | - |
Gaeumannomyces graminis |
Mn-LOX | - |
Gaeumannomyces graminis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
linoleate | pH 7.5, 18:3n-3 substrate | Gaeumannomyces graminis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Gaeumannomyces graminis |