Cloned (Comment) | Organism |
---|---|
expression of wild-type and G316A mutant enzymes in Pichia pastoris strain X-33, secretion to the cell medium | Gaeumannomyces graminis |
Protein Variants | Comment | Organism |
---|---|---|
G316A | site-directed mutagenesis, the mutant MnIII-LO G316A oxygenates mainly 16:3n-3 at positions n-7 and n-6, 19:3n-3 at n-10, n-8, and n-6, and 20:3n-3 at n-10 and n-8 | Gaeumannomyces graminis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | a Mn-lipoxygenase | Gaeumannomyces graminis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gaeumannomyces graminis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Pichia pastoris cell medium by hydrophobic interaction and ion exchange chromatography | Gaeumannomyces graminis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
linoleate + O2 = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate | reaction mechanism, tail-first model with rearrangement at C13 at the end of lipoxygenation, influenced by double bond configuration and the chain length of fatty acids, overview | Gaeumannomyces graminis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(10Z,13Z,16Z)-nonadeca-10,13,16-trienoic acid + O2 | oxidation at the n-6 carbon and at the bis-allylic position n-8 | Gaeumannomyces graminis | ? | - |
? | |
(11Z,14Z,17Z)-eicosa-11,14,17-trienoic acid + O2 | oxidation at the n-6 carbon and at the bis-allylic position n-8 | Gaeumannomyces graminis | ? | - |
? | |
(5E,8E,11E,14E)-icosa-5,8,11,14-tetraenoic acid + O2 | oxidation at the n-6 carbon and at the bis-allylic position n-8 | Gaeumannomyces graminis | (5Z,8Z,11S,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8Z,11Z,13E,15R)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid | - |
? | |
(5Z,8Z,11Z,14Z,17Z)-docosa-5,8,11,14,17-pentaenoate + O2 | oxidation at the n-6 carbon and at the bis-allylic position n-8 | Gaeumannomyces graminis | ? | - |
? | |
(7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoate + O2 | oxidation at the n-6 carbon and at the bis-allylic position n-8 | Gaeumannomyces graminis | ? | - |
? | |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | via bis-allylic 11S-hydroperoxy fatty acid | Gaeumannomyces graminis | (13R)-hydroperoxyoctadecadienoic acid | - |
? | |
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2 | oxidation at the bis-allylic n-5 carbon and at positions n-3, n-7, and n-6 | Gaeumannomyces graminis | ? | - |
? | |
1,2-dilinoleoyl-sn-glycero-3-phosphocholine + O2 | - |
Gaeumannomyces graminis | ? | main products with 14% and 10%, no production of 11-hydroxyoctadecanoic acid | ? | |
linoleate + O2 | via bis-allylic 11S-hydroperoxy fatty acid | Gaeumannomyces graminis | (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
additional information | Mn-LO likely binds fatty acids tail-first and oxygenates many C16, C18, C20, and C22 fatty acids to significant amounts of bis-allylic hydroperoxides. Unsaturated C16-C22 fatty acids, other than 18:3n-3, 18:2n-6, or 17:3n-3, are poor substrates, possibly because of ineffective enzyme activation through Mn2+ to Mn3+ by the produced hydroperoxides, substrate specificities of wild-type and mutant enzymes, overview | Gaeumannomyces graminis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
manganese lipoxygenase | - |
Gaeumannomyces graminis |
Mn-LO | - |
Gaeumannomyces graminis |