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Literature summary for 1.13.11.45 extracted from

  • Koch, R.B.; Brumfiel, B.L.; Brumfiel, M.N.
    Calcium requirement for lipoxygenase catalyzed linoleate oxidation (1971), J. Am. Oil Chem. Soc., 48, 532-538.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Tween 20 inhibition of Ca2+-activated enzyme at pH 7.5 and 6.4, no inhibition at pH 6.4 in the absence of Ca2+ Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximum activity between 0.24 and 0.92 mM in phosphate buffer Phaseolus vulgaris
Ca2+ maximum activity between 0.24 and 0.49 mM in phosphate buffer Glycine max
Mg2+ activation less effective than with Ca2+ in phosphate buffer Phaseolus vulgaris
Mn2+ activation less effective than with Ca2+ in phosphate buffer Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Glycine max
-
soy bean
-
Phaseolus vulgaris
-
navy bean
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + O2
-
Phaseolus vulgaris (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
?
linoleic acid + O2
-
Glycine max (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
in the absence of Ca2+ Phaseolus vulgaris
7.2
-
in the presence of 0.37 mM Ca2+ Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4 9 in the presence of 0.37 mM Ca2+ Phaseolus vulgaris
4 7 in the absence of Ca2+ Phaseolus vulgaris