Cloned (Comment) | Organism |
---|---|
genes nagI1, nagI2, and nagI3, the latter encoded in the third cluster, nagl3 is polycistronic and is expressed at a relatively high level, genetic organization, DNA and amino acid sequence determination and analysis, real-time RT-PCR for gene expression analysis of genes nagI1, nagI2 and nagI3 and the third catabolic gene cluster, overview. Overexpression of all three genes in Escherichia coli strain BL21(DE3). The transformation efficiency of the pET21bnagI1 ligation product is very low compared with that of the nagI2 and nagI3 genes | Polaromonas naphthalenivorans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of individual knockout mutants of all three nagI genes via 2 bp unmarked gene deletion mutagenesis, all three nagI knockout mutants still contain stop codons within their nagI genes. Mutant CJ2DELTAnagI3 shows severely diminished GDO activity and grows slowest on aromatic substrates | Polaromonas naphthalenivorans |
General Stability | Organism |
---|---|
purified GDO proteins NagI2 and NagI3 are highly unstable in phosphate buffer without ferrous ions | Polaromonas naphthalenivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | purified GDO proteins NagI2 and NagI3 are highly unstable in phosphate buffer without ferrous ions | Polaromonas naphthalenivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | Polaromonas naphthalenivorans | - |
maleylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Polaromonas naphthalenivorans | - |
three nagl genes, nagI1, nagI2, and nagI3, the latter belongs to the third cluster | - |
Purification (Comment) | Organism |
---|---|
recombinant Nagl2 and Nagl3 isozymes from Escherichia coli strain BL21(DE3) by gel filtration and sequential anion exchange chromatography | Polaromonas naphthalenivorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:naphthalene-grown cell | - |
Polaromonas naphthalenivorans | - |
additional information | growth rate of wild-type strain CJ2 and three nagI knockout mutants on six different agar media, overview | Polaromonas naphthalenivorans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Polaromonas naphthalenivorans | maleylpyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000-39000 | Polaromonas naphthalenivorans |
Synonyms | Comment | Organism |
---|---|---|
GDO | - |
Polaromonas naphthalenivorans |
Nagl1 | - |
Polaromonas naphthalenivorans |
Nagl2 | - |
Polaromonas naphthalenivorans |
Nagl3 | - |
Polaromonas naphthalenivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
isozyme Nagl2 | Polaromonas naphthalenivorans |
65 | - |
isozyme Nagl3 | Polaromonas naphthalenivorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | isozyme Nagl2, 74.1% activity at 25°C, maximum activity at 50°C | Polaromonas naphthalenivorans |
25 | 65 | isozyme Nagl4, 72.4% activity at 25°C, maximum activity at 65°C | Polaromonas naphthalenivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
isozyme Nagl3 | Polaromonas naphthalenivorans |
8 | - |
isozyme Nagl2 | Polaromonas naphthalenivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.4 | activity range, isozymes Nagl2 and Nagl3 | Polaromonas naphthalenivorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Polaromonas naphthalenivorans | about, isozymes Nagl2 and Nagl3, isoelectric focusing | - |
5.4 |
Polaromonas naphthalenivorans | isozyme Nagl1, sequence calculation | - |
6.8 |
Organism | Comment | Expression |
---|---|---|
Polaromonas naphthalenivorans | nagl3 gene expression is induced by salicylate in wild-type strain CJ2 but not in the nagR knockout mutant strain CJN110 | up |
General Information | Comment | Organism |
---|---|---|
metabolism | strain CJ2 metabolizes naphthalene via the gentisate pathway using gentisate 1,2-dioxygenase, patterns in regulation, transcription and enzyme activity, overview. The nagR regulator controls the third catabolic gene cluster | Polaromonas naphthalenivorans |
physiological function | although all three nagI genes in strain CJ2 influence naphthalene degradation, the nagI3 gene plays the most significant role. NagI3 may prevent toxic intracellular levels of gentisate from accumulating in CJ2 cells | Polaromonas naphthalenivorans |