Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Xanthobacter polyaromaticivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hydroxy 2 naphthoate | 0.33 mM, 19% of wild type enzyme activity remaining | Xanthobacter polyaromaticivorans | |
Fe | 2 mM, complete inactivation | Xanthobacter polyaromaticivorans | |
salicylate | 0.33 mM, 38% of wild type enzyme activity remaining | Xanthobacter polyaromaticivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0186 | - |
2,5-Dihydroxybenzoate | - |
Xanthobacter polyaromaticivorans | |
0.0209 | - |
1-hydroxy 2 naphthoate | - |
Xanthobacter polyaromaticivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | ferrous ion specifically functions in the formation of the active tetramer structure, the enzyme contains 0.98 mol iron per mol subunit | Xanthobacter polyaromaticivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
4 * 36500, gel filtration | Xanthobacter polyaromaticivorans |
39300 | - |
calculated from amino acid sequence | Xanthobacter polyaromaticivorans |
40000 | - |
SDS-PAGE | Xanthobacter polyaromaticivorans |
146000 | - |
gel filtration | Xanthobacter polyaromaticivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter polyaromaticivorans | Q75W71 | - |
- |
Xanthobacter polyaromaticivorans 127W | Q75W71 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrapQ column chromatography | Xanthobacter polyaromaticivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the purified recombinant enzyme is 102 units/mg at pH 7.5 | Xanthobacter polyaromaticivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-dihydroxy-2-naphthoate + O2 | - |
Xanthobacter polyaromaticivorans | ? | - |
? | |
1,4-dihydroxy-2-naphthoate + O2 | - |
Xanthobacter polyaromaticivorans 127W | ? | - |
? | |
1-hydroxy-2-naphthoate + O2 | weak activity | Xanthobacter polyaromaticivorans | ? | - |
? | |
1-hydroxy-2-naphthoate + O2 | weak activity | Xanthobacter polyaromaticivorans 127W | ? | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Xanthobacter polyaromaticivorans | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Xanthobacter polyaromaticivorans 127W | maleylpyruvate | - |
? | |
5-aminosalicylate + O2 | partly preferred substrate | Xanthobacter polyaromaticivorans | ? | - |
? | |
5-aminosalicylate + O2 | partly preferred substrate | Xanthobacter polyaromaticivorans 127W | ? | - |
? | |
additional information | no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate | Xanthobacter polyaromaticivorans | ? | - |
? | |
additional information | no activity against catechol, benzoate, 5-methylsalicylate, 5-chlorosalicylate, and homogentisate | Xanthobacter polyaromaticivorans 127W | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36500, gel filtration | Xanthobacter polyaromaticivorans |
Synonyms | Comment | Organism |
---|---|---|
GDO | - |
Xanthobacter polyaromaticivorans |
gentisate dioxygenase | - |
Xanthobacter polyaromaticivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme loses almost all its activity after incubation at 50°C for 15 min | Xanthobacter polyaromaticivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.565 | - |
1-hydroxy 2 naphthoate | - |
Xanthobacter polyaromaticivorans | |
5.5 | - |
2,5-Dihydroxybenzoate | - |
Xanthobacter polyaromaticivorans | |
6.08 | - |
1-hydroxy 2 naphthoate | - |
Xanthobacter polyaromaticivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
GDO loses its activity after being kept in buffer below pH 6.5 for 12 h in the absence of ferrous iron and cysteine | Xanthobacter polyaromaticivorans |
8 | - |
GDO loses its activity after being kept in buffer above pH 8.0 for 12 h in the absence of ferrous iron and cysteine | Xanthobacter polyaromaticivorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Xanthobacter polyaromaticivorans | calculated from amino acid sequence | - |
5.4 |