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Literature summary for 1.13.11.4 extracted from
- Gentisate 1,2-dioxygenase from Pseudomonas acidovorans (1990), Methods Enzymol., 188, 101-107.
General Stability
| General Stability | Organism |
|---|---|
| cysteine stabilizes | Delftia acidovorans |
| Fe2+ stabilizes | Delftia acidovorans |
| glycerol stabilizes | Delftia acidovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-hydroxybenzoate | - |
Delftia acidovorans |  |
| salicylate | - |
Delftia acidovorans | |
| Thiosalicylate | - |
Delftia acidovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Delftia acidovorans | |
| 0.055 | - |
O2 | - |
Delftia acidovorans | |
| 0.08 | - |
gentisate | - |
Delftia acidovorans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delftia acidovorans | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| exposure of enzyme to oxidants, e.g. H2O2 or K3Fe(CN)6, at 1 mM concentrations results in complete loss of activity, even under anaerobic conditions | Delftia acidovorans |
| under turnover conditions enzyme is rapidly inactivated at O2 concentrations above 0.8 mM | Delftia acidovorans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Delftia acidovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dihydroxybenzoate + O2 | - |
Delftia acidovorans | maleylpyruvate | - |
? | |
| additional information | broad substrate specificity | Delftia acidovorans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37200-39800, SDS-PAGE | Delftia acidovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 322 | 328 | gentisate | - |
Delftia acidovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Delftia acidovorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Delftia acidovorans |
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Release 2023.1 (January 2023)
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