Cloned (Comment) | Organism |
---|---|
gene bphC, recombinant expression of His-tagged enzyme in Escherichia coli strain SG13009 | Delftia acidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten constants derived from LineweaverBurk plots | Delftia acidovorans | |
0.0117 | - |
biphenyl-2,3-diol | pH 9.0, 25°C, recombinant His-tagged enzyme | Delftia acidovorans | |
0.024 | - |
3-methylcatechol | pH 9.0, 25°C, recombinant His-tagged enzyme | Delftia acidovorans | |
0.625 | - |
catechol | pH 9.0, 25°C, recombinant His-tagged enzyme | Delftia acidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Delftia acidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration, recombinant His-tagged enzyme | Delftia acidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Delftia acidovorans | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | Delftia acidovorans SMN4 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | Q8KRD4 | - |
- |
Delftia acidovorans SMN4 | Q8KRD4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Delftia acidovorans | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Delftia acidovorans SMN4 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Delftia acidovorans | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Delftia acidovorans SMN4 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Delftia acidovorans | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Delftia acidovorans SMN4 | 2-hydroxymuconate semialdehyde | - |
? | |
additional information | 4-methylcatechol is a poor substrate for the enzyme | Delftia acidovorans | ? | - |
? | |
additional information | 4-methylcatechol is a poor substrate for the enzyme | Delftia acidovorans SMN4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 33000, recombinant His-tagged enzyme, SDS-PAGE, x * 34285, His-tagged enzyme, sequence calculation | Delftia acidovorans |
Synonyms | Comment | Organism |
---|---|---|
2,3-DBDO | - |
Delftia acidovorans |
2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Delftia acidovorans |
BphC | - |
Delftia acidovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Delftia acidovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged enzyme, stable up to for 24 h | Delftia acidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Delftia acidovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | purified recombinant His-tagged enzyme, stability range of alpha-helical folding structured enzyme, inactive unfolded enzyme at pH 2.5and pH 12.0, most stable at pH 8.0. At pH 7.5 stable for 24 h | Delftia acidovorans |
General Information | Comment | Organism |
---|---|---|
metabolism | 2,3-dihydroxybiphenyl 1,2-dioxygenase is an extradiol-type dioxygenase involved in third step of biphenyl degradation pathway | Delftia acidovorans |