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Literature summary for 1.13.11.39 extracted from

  • Cao, L.; Gao, Y.; Wu, G.; Li, M.; Xu, J.; He, J.; Li, S.; Hong, Q.
    Cloning of three 2,3-dihydroxybiphenyl-1,2-dioxygenase genes from Achromobacter sp. BP3 and the analysis of their roles in the biodegradation of biphenyl (2013), J. Hazard. Mater., 261, 246-252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bphC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.
gene bphC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.
gene bphC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Achromobacter sp.
Co2+
-
Achromobacter sp.
Cu2+
-
Achromobacter sp.
Fe2+
-
Achromobacter sp.
Fe3+ slight inhibition Achromobacter sp.
Mg2+
-
Achromobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
0.00213
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
0.0049
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
0.0062
-
catechol pH 7.5, 30°C Achromobacter sp.
0.01672
-
catechol pH 7.5, 30°C Achromobacter sp.
0.0273
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
0.0391
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
0.0616
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
0.0976
-
catechol pH 7.5, 30°C Achromobacter sp.
0.1306
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
0.2824
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
0.3605
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates 270% Achromobacter sp.
additional information no effect by Fe3+, Mg2+ and Ca2+ Achromobacter sp.
additional information no effect by Fe3+, Mg2+ and Ca2+, poor activating effect by Fe2+ Achromobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Achromobacter sp.
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2 Achromobacter sp. BP3
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
isolated from petroleum-contaminated soil, gene bphC3
-
Achromobacter sp. B3VTK1 BphC1; isolated from petroleum-contaminated soil, gene bphC1
-
Achromobacter sp. B3VTL5 BphC2; isolated from petroleum-contaminated soil, gene bphC2
-
Achromobacter sp. BP3
-
isolated from petroleum-contaminated soil, gene bphC3
-
Achromobacter sp. BP3 B3VTK1 BphC1; isolated from petroleum-contaminated soil, gene bphC1
-
Achromobacter sp. BP3 B3VTL5 BphC2; isolated from petroleum-contaminated soil, gene bphC2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
Achromobacter sp. ?
-
?
3-methylcatechol + O2
-
Achromobacter sp. BP3 ?
-
?
4-methylcatechol + O2
-
Achromobacter sp. ?
-
?
4-methylcatechol + O2
-
Achromobacter sp. BP3 ?
-
?
biphenyl-2,3-diol + O2
-
Achromobacter sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2 highly preferred substrate Achromobacter sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2
-
Achromobacter sp. BP3 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
biphenyl-2,3-diol + O2 highly preferred substrate Achromobacter sp. BP3 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
?
catechol + O2
-
Achromobacter sp. cis,cis-muconate
-
?
catechol + O2
-
Achromobacter sp. BP3 cis,cis-muconate
-
?

Synonyms

Synonyms Comment Organism
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
Achromobacter sp.
BphC
-
Achromobacter sp.
BphC1
-
Achromobacter sp.
BphC2
-
Achromobacter sp.
BphC3
-
Achromobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Achromobacter sp.
40
-
-
Achromobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, profile overview Achromobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.19
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
3.25
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
7.36
-
catechol pH 7.5, 30°C Achromobacter sp.
8.78
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
9.87
-
catechol pH 7.5, 30°C Achromobacter sp.
10.15
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
12.02
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
21.63
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
43.2
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
52.56
-
catechol pH 7.5, 30°C Achromobacter sp.
53.83
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
86.32
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Achromobacter sp.
8 9
-
Achromobacter sp.
9
-
-
Achromobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile overview Achromobacter sp.

Expression

Organism Comment Expression
Achromobacter sp. gene bphC2 is constitutively expressed in strain BP3 up
Achromobacter sp. gene bphC3 is induced by both biphenyl and toluene up
Achromobacter sp. the bphC1 gene is only induced by biphenyl up

General Information

General Information Comment Organism
metabolism BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview Achromobacter sp.
metabolism BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview Achromobacter sp.
metabolism BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview Achromobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.21
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
9
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
92
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
101.1
-
catechol pH 7.5, 30°C Achromobacter sp.
164.8
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
224.6
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
440.2
-
catechol pH 7.5, 30°C Achromobacter sp.
1582
-
4-methylcatechol pH 7.5, 30°C Achromobacter sp.
8477
-
catechol pH 7.5, 30°C Achromobacter sp.
10300
-
3-methylcatechol pH 7.5, 30°C Achromobacter sp.
10990
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
40530
-
biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.