Cloned (Comment) | Organism |
---|---|
gene bphC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
gene bphC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
gene bphC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 | Achromobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Achromobacter sp. | |
Co2+ | - |
Achromobacter sp. | |
Cu2+ | - |
Achromobacter sp. | |
Fe2+ | - |
Achromobacter sp. | |
Fe3+ | slight inhibition | Achromobacter sp. | |
Mg2+ | - |
Achromobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
0.00213 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
0.0049 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
0.0062 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
0.01672 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
0.0273 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
0.0391 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
0.0616 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
0.0976 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
0.1306 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
0.2824 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
0.3605 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates 270% | Achromobacter sp. | |
additional information | no effect by Fe3+, Mg2+ and Ca2+ | Achromobacter sp. | |
additional information | no effect by Fe3+, Mg2+ and Ca2+, poor activating effect by Fe2+ | Achromobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Achromobacter sp. | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | Achromobacter sp. BP3 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | - |
isolated from petroleum-contaminated soil, gene bphC3 | - |
Achromobacter sp. | B3VTK1 | BphC1; isolated from petroleum-contaminated soil, gene bphC1 | - |
Achromobacter sp. | B3VTL5 | BphC2; isolated from petroleum-contaminated soil, gene bphC2 | - |
Achromobacter sp. BP3 | - |
isolated from petroleum-contaminated soil, gene bphC3 | - |
Achromobacter sp. BP3 | B3VTK1 | BphC1; isolated from petroleum-contaminated soil, gene bphC1 | - |
Achromobacter sp. BP3 | B3VTL5 | BphC2; isolated from petroleum-contaminated soil, gene bphC2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Achromobacter sp. | ? | - |
? | |
3-methylcatechol + O2 | - |
Achromobacter sp. BP3 | ? | - |
? | |
4-methylcatechol + O2 | - |
Achromobacter sp. | ? | - |
? | |
4-methylcatechol + O2 | - |
Achromobacter sp. BP3 | ? | - |
? | |
biphenyl-2,3-diol + O2 | - |
Achromobacter sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | highly preferred substrate | Achromobacter sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Achromobacter sp. BP3 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | highly preferred substrate | Achromobacter sp. BP3 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Achromobacter sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Achromobacter sp. BP3 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Achromobacter sp. |
BphC | - |
Achromobacter sp. |
BphC1 | - |
Achromobacter sp. |
BphC2 | - |
Achromobacter sp. |
BphC3 | - |
Achromobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Achromobacter sp. |
40 | - |
- |
Achromobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | activity range, profile overview | Achromobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.19 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
3.25 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
7.36 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
8.78 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
9.87 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
10.15 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
12.02 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
21.63 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
43.2 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
52.56 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
53.83 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
86.32 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Achromobacter sp. |
8 | 9 | - |
Achromobacter sp. |
9 | - |
- |
Achromobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile overview | Achromobacter sp. |
Organism | Comment | Expression |
---|---|---|
Achromobacter sp. | gene bphC2 is constitutively expressed in strain BP3 | up |
Achromobacter sp. | gene bphC3 is induced by both biphenyl and toluene | up |
Achromobacter sp. | the bphC1 gene is only induced by biphenyl | up |
General Information | Comment | Organism |
---|---|---|
metabolism | BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview | Achromobacter sp. |
metabolism | BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview | Achromobacter sp. |
metabolism | BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview | Achromobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.21 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
9 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
92 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
101.1 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
164.8 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
224.6 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
440.2 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
1582 | - |
4-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
8477 | - |
catechol | pH 7.5, 30°C | Achromobacter sp. | |
10300 | - |
3-methylcatechol | pH 7.5, 30°C | Achromobacter sp. | |
10990 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. | |
40530 | - |
biphenyl-2,3-diol | pH 7.5, 30°C | Achromobacter sp. |