Cloned (Comment) | Organism |
---|---|
gene BsbphCI, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, the transgenic Escherichia coli strain expressing the BsbphCI gene degrades biphenyl-2,3-diol more quickly than the wild-type strain | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
Cr3+ | - |
Bacillus sp. (in: Bacteria) | |
Fe2+ | - |
Bacillus sp. (in: Bacteria) | |
H2O2 | 81% inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
KI | 43% inhibition at 600 mM | Bacillus sp. (in: Bacteria) | |
Li+ | - |
Bacillus sp. (in: Bacteria) | |
SDS | 46% inhibition at 25 mM | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates | Bacillus sp. (in: Bacteria) | |
Co2+ | activates | Bacillus sp. (in: Bacteria) | |
Cu2+ | activates | Bacillus sp. (in: Bacteria) | |
K+ | activates | Bacillus sp. (in: Bacteria) | |
Mg2+ | activates | Bacillus sp. (in: Bacteria) | |
Ni2+ | activates | Bacillus sp. (in: Bacteria) | |
Zn2+ | activates | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, recombinant enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Bacillus sp. (in: Bacteria) | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | Bacillus sp. (in: Bacteria) JF8 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q8GR45 | gene BsbphCI | - |
Bacillus sp. (in: Bacteria) JF8 | Q8GR45 | gene BsbphCI | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | - |
Bacillus sp. (in: Bacteria) | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, recombinant enzyme, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
2,3-DHBP dioxygenase | - |
Bacillus sp. (in: Bacteria) |
2,3-dihydroxybiphenyl-1,2-dioxygenase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, 60 min, 33% activity remaining | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |
General Information | Comment | Organism |
---|---|---|
physiological function | 2,3-dihydroxybiphenyl-1,2-dioxygenase plays an important role in the degradation of polychlorinated biphenyls | Bacillus sp. (in: Bacteria) |