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Literature summary for 1.13.11.39 extracted from

  • McKay, D.B.; Prucha, M.; Reineke, W.; Timmis, K.N.; Pieper, D.H.
    Substrate specificity and expression of three 2,3-dihydroxybiphenyl 1,2-dioxygenases from Rhodococcus globerulus strain P6 (2003), J. Bacteriol., 185, 2944-2951.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00045
-
3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.001
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.001
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.0015
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.01
-
3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.011
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.012
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.018
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.019
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.026
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.029
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.1
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.23
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
1.94
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
2.4
-
catechol 25°C, pH 7.5 Rhodococcus globerulus
2.8
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
61
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
93
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Rhodococcus globerulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, SDS-PAGE Rhodococcus globerulus
29000
-
x * 29000, SDS-PAGE Rhodococcus globerulus
34000
-
x * 34000, SDS-PAGE Rhodococcus globerulus

Organism

Organism UniProt Comment Textmining
Rhodococcus globerulus
-
-
-
Rhodococcus globerulus P6
-
-
-

Oxidation Stability

Oxidation Stability Organism
50% loss of activity after 4.5 min in air-satured buffer Rhodococcus globerulus
50% loss of activity after 43 min in air-satured buffer Rhodococcus globerulus
50% loss of activity after 7.2 min in air-satured buffer Rhodococcus globerulus

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus globerulus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.5
-
-
Rhodococcus globerulus
20
-
-
Rhodococcus globerulus
30
-
-
Rhodococcus globerulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 25% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 57% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 25% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 57% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',4'-dichloro-2,3-dihydroxybiphenyl + O2 33% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2',4'-dichloro-2,3-dihydroxybiphenyl + O2 63% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2'-chloro-2,3-dihydroxybiphenyl + O2
-
Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2'-chloro-2,3-dihydroxybiphenyl + O2 50% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
2,3-dihydroxybiphenyl + O2
-
Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 11% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 26% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 59% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 15% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 49% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3'-chloro-2,3-dihydroxybiphenyl + O2
-
Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3'-chloro-2,3-dihydroxybiphenyl + O2 64% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3'-chloro-2,3-dihydroxybiphenyl + O2 71% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3'-chloro-2,3-dihydroxybiphenyl + O2 95% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
3-methylcatechol + O2
-
Rhodococcus globerulus ?
-
?
3-methylcatechol + O2
-
Rhodococcus globerulus P6 ?
-
?
4'-chloro-2,3-dihydroxybiphenyl + O2
-
Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
4'-chloro-2,3-dihydroxybiphenyl + O2 61% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
4'-chloro-2,3-dihydroxybiphenyl + O2 77% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
4'-chloro-2,3-dihydroxybiphenyl + O2 84% of activity with 2,3-dihydroxybiphenyl Rhodococcus globerulus 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
-
?
4-methylcatechol + O2
-
Rhodococcus globerulus ?
-
?
catechol + O2
-
Rhodococcus globerulus ?
-
?
catechol + O2
-
Rhodococcus globerulus P6 ?
-
?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Rhodococcus globerulus
? x * 34000, SDS-PAGE Rhodococcus globerulus
? x * 29000, SDS-PAGE Rhodococcus globerulus

Synonyms

Synonyms Comment Organism
BphC1
-
Rhodococcus globerulus
BphC2
-
Rhodococcus globerulus
BphC3
-
Rhodococcus globerulus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
catechol 25°C, pH 7.5 Rhodococcus globerulus
0.064
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
0.24
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
0.25
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
0.36
-
4-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
0.92
-
catechol 25°C, pH 7.5 Rhodococcus globerulus
3.5
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
4
-
3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
4.4
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
4.5
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
4.8
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
7.5
-
2'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
8.3
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
8.5
-
3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
9.8
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
9.8
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
12
-
3'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
13.6
-
3-methylcatechol 25°C, pH 7.5 Rhodococcus globerulus
16.5
-
2,3-Dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus
18.8
-
4'-chloro-2,3-dihydroxybiphenyl 25°C, pH 7.5 Rhodococcus globerulus