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Literature summary for 1.13.11.39 extracted from

  • Khan, A.A.; Wang, R.F.; Nawaz, M.S.; Cao, W.W.; Cerniglia, C.E.
    Purification of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Pseudomonas putida OU83 and characterization of the gene (bphC) (1996), Appl. Environ. Microbiol., 62, 1825-1830.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas putida
-
 Comamonas testosteroni
strain OU83, gene bphC, strain OU83, 2 different 2,3-DBPDs cloned with plasmid pAW590 and expressed in Escherichia coli JM109 Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 2,3-Dihydroxybiphenyl strain OU83 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Pseudomonas sp.
Fe2+
-
 Comamonas testosteroni
Fe2+ significantly enhances activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31900
-
 8 * 31900, nucleotide sequence Pseudomonas putida
32000
-
 8 * 32000, SDS-PAGE Pseudomonas putida
256000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Pseudomonas aeruginosa
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas putida
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Comamonas testosteroni
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Burkholderia cepacia
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas oleovorans
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Sphingobium yanoikuyae
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Rhodococcus sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas sp. IC
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas oleovorans KF714
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas putida PpG7
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Rhodococcus sp. RHA1
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas oleovorans KF707
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas putida OU83
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas putida KF715
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
 organism reclassified, prior refered as Pseudomonas sp. strain LB400 or Pseudomonas cepacia strain LB400
-
Comamonas testosteroni
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas oleovorans
-
-
-
Pseudomonas oleovorans KF707
-
-
-
Pseudomonas oleovorans KF714
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida Q52032
-
-
Pseudomonas putida KF715
-
-
-
Pseudomonas putida OU83 Q52032
-
-
Pseudomonas putida PpG7
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. IC
-
-
-
Rhodococcus sp. Q53126
-
-
Rhodococcus sp. RHA1 Q53126
-
-
Sphingobium yanoikuyae
-
 formerly Pseudomonas paucimobilis strain Q1
-
Sphingobium yanoikuyae
-
 strain B1, formerly Beijerincka sp. wild-type strain B1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
380
-
-
 Pseudomonas putida

Storage Stability

Storage Stability Organism
25°C, 30% activity lost when stored for 8 h Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Sphingobium yanoikuyae 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Rhodococcus sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas sp. IC 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas oleovorans KF714 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas putida PpG7 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Rhodococcus sp. RHA1 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas oleovorans KF707 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2
-
 Pseudomonas putida KF715 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-chloro-2,3-dihydroxybiphenyl + O2
-
 Comamonas testosteroni 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
4-chloro-2,3-dihydroxybiphenyl + O2
-
 Pseudomonas putida 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
4-chloro-2,3-dihydroxybiphenyl + O2
-
 Pseudomonas putida OU83 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
4-chlorobiphenyl + O2
-
 Comamonas testosteroni ?
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas aeruginosa 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Comamonas testosteroni 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Sphingobium yanoikuyae 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Rhodococcus sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas sp. IC 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans KF714 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas putida PpG7 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Rhodococcus sp. RHA1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans KF707 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas putida OU83 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas putida KF715 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
catechol + O2
-
 Pseudomonas sp. ?
-
 ?
catechol + O2
-
 Pseudomonas putida ?
-
 ?
catechol + O2
-
 Burkholderia cepacia ?
-
 ?
catechol + O2
-
 Pseudomonas oleovorans ?
-
 ?
catechol + O2
-
 Sphingobium yanoikuyae ?
-
 ?
catechol + O2
-
 Rhodococcus sp. ?
-
 ?
catechol + O2
-
 Pseudomonas sp. IC ?
-
 ?
catechol + O2
-
 Pseudomonas oleovorans KF714 ?
-
 ?
catechol + O2
-
 Pseudomonas putida PpG7 ?
-
 ?
catechol + O2
-
 Rhodococcus sp. RHA1 ?
-
 ?
catechol + O2
-
 Pseudomonas oleovorans KF707 ?
-
 ?
catechol + O2
-
 Pseudomonas putida OU83 ?
-
 ?
catechol + O2
-
 Pseudomonas putida KF715 ?
-
 ?
additional information 3,4-dihydroxybiphenyl is no substrate Pseudomonas putida ?
-
 ?
additional information 3,4-dihydroxybiphenyl is no substrate Pseudomonas putida PpG7 ?
-
 ?
additional information 3,4-dihydroxybiphenyl is no substrate Pseudomonas putida KF715 ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 32000, SDS-PAGE Pseudomonas putida
octamer 8 * 31900, nucleotide sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
2,3-DBPD
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 enzyme loses catalytic activity when heated for 10 min Pseudomonas oleovorans