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Literature summary for 1.13.11.37 extracted from

  • Latus, M.; Seitz, H.J.; Eberspaecher, J.; Lingens, F.
    Purification and characterization of hydroxyquinol 1,2-dioxygenase from Azotobacter sp. strain GP1 (1995), Appl. Environ. Microbiol., 61, 2453-2460.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.37

Activating Compound

Activating Compound Comment Organism Structure
Fe(NH4)2(SO4)2 0.00001-0.000001 mM Azotobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Tiron
-
 Azotobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 0.18 mol per mol enzyme Azotobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34250
-
 2 * 34250, SDS-PAGE Azotobacter sp.
58000
-
 gel filtration Azotobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzene-1,2,4-triol + O2 Azotobacter sp.
-
 3-hydroxy-cis-cis-muconate
-
 ?
benzene-1,2,4-triol + O2 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
 3-hydroxy-cis-cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter sp.
-
 grown with 2,4,6-trichlorophenol
-
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
 grown with 2,4,6-trichlorophenol
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.06
-
 reactivated with 0.01 mM Fe2+ Azotobacter sp.

Storage Stability

Storage Stability Organism
-80°C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day Azotobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-chlorohydroxyquinol + O2 33.5% relative activity of that measured with hydroxyquinol Azotobacter sp. 2-chloromaleylacetate
-
 ?
6-chlorohydroxyquinol + O2 33.5% relative activity of that measured with hydroxyquinol Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 2-chloromaleylacetate
-
 ?
benzene-1,2,4-triol + O2
-
 Azotobacter sp. maleylacetate maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate ?
benzene-1,2,4-triol + O2
-
 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 maleylacetate maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate ?
benzene-1,2,4-triol + O2
-
 Azotobacter sp. 3-hydroxy-cis-cis-muconate
-
 ?
benzene-1,2,4-triol + O2
-
 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 3-hydroxy-cis-cis-muconate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34250, SDS-PAGE Azotobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 in succinate buffer Azotobacter sp.