Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star cells | Crocosphaera subtropica ATCC 51142 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | LOX2 contains iron with an iron to protein ratio of 45% | Crocosphaera subtropica ATCC 51142 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Crocosphaera subtropica ATCC 51142 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crocosphaera subtropica ATCC 51142 | B7JX99 | - |
- |
Crocosphaera subtropica ATCC 51142 PCC 8801 | B7JX99 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, gel filtration | Crocosphaera subtropica ATCC 51142 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diarachidonoylglycerophosphocholine + O2 | - |
Crocosphaera subtropica ATCC 51142 | ? | - |
? | |
1,2-diarachidonoylglycerophosphocholine + O2 | - |
Crocosphaera subtropica ATCC 51142 PCC 8801 | ? | - |
? | |
arachidonic acid + O2 | - |
Crocosphaera subtropica ATCC 51142 | 13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid | - |
? | |
arachidonic acid + O2 | - |
Crocosphaera subtropica ATCC 51142 PCC 8801 | 13-hydroperoxyeicosatetraenoic acid + (5Z,8Z,11R,12E,14Z)-11-hydroperoxyicosa-5,8,12,14-tetraenoic acid + (5Z,8E,11Z,13E,15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoic acid | - |
? | |
linoleate + O2 | - |
Crocosphaera subtropica ATCC 51142 | (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate | (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product | ? | |
linoleate + O2 | - |
Crocosphaera subtropica ATCC 51142 PCC 8801 | (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate + (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate + (9R,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate | (9Z,11R,12Z)-11-hydroperoxyoctadeca-9,12-dienoate is the primary product | ? | |
linolenate + O2 | - |
Crocosphaera subtropica ATCC 51142 | 11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid | - |
? | |
linolenate + O2 | - |
Crocosphaera subtropica ATCC 51142 PCC 8801 | 11-hydroperoxyoctadecatrienoic acid + 9-hydroperoxyoctadecatrienoic acid + 13-hydroperoxyoctadecatrienoic acid | - |
? | |
additional information | incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase | Crocosphaera subtropica ATCC 51142 | ? | - |
? | |
additional information | incubation of the enzyme with [(11S)-2H]-linoleic acid leads to the formation of hydroperoxides that have lost the deuterium label, thus suggesting that LOX2 catalyzes antarafacial oxygenation as opposed to the mechanism of manganese lipoxygenase | Crocosphaera subtropica ATCC 51142 PCC 8801 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LOX2 | - |
Crocosphaera subtropica ATCC 51142 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Crocosphaera subtropica ATCC 51142 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Crocosphaera subtropica ATCC 51142 |