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Literature summary for 1.13.11.33 extracted from

  • Wecksler, A.T.; Kenyon, V.; Deschamps, J.D.; Holman, T.R.
    Substrate specificity changes for human reticulocyte and epithelial 15-lipoxygenases reveal allosteric product regulation (2008), Biochemistry, 47, 7364-7375.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme 15-hLO-1 as His-tagged enzyme, and of untagged isozyme 15-hLO-2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics of isozymes 15-hLO-1 and 15-hLO-2 with arachidonate and linoleic acid in presence or absence of 0.2% sodium cholate, or of oxygenated fatty acids, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, iron content of the 15-hLO isozymes determined with a Finnigan inductively coupled plasma mass spectrometer, ICP-MS, using cobalt-EDTA as an internal standard Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + O2 Homo sapiens
-
 (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?
linoleic acid + O2 Homo sapiens
-
 (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes 15-hLO-1 and 15-hLO-2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme 15-hLO-1 by nickel affinity chromatography, recombinant untagged isozyme 15-hLO-2 by ion exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell isozyme 15-hLO-2 Homo sapiens
-
reticulocyte isozyme 15-hLO-1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?
arachidonate + O2 preferred substrate of isozyme 15-hLO-2, reaction of EC 1.13.11.33 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?
linoleic acid + O2
-
 Homo sapiens (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate
-
 ?
linoleic acid + O2 preferred substrate of isozyme 15-hLO-1, reaction of EC 1.13.11.12 Homo sapiens (9Z,11E)-(13S)-13-hydroperoxy-octadeca-9,11-dienoate
-
 ?
additional information substrate specificity of 15-hLO isozymes, the specific activity is affected by cholate and lipoxygenation reaction products, e.g. 13-hydroperoxyoctadienoic acid changes the (kcat/Km)AA/(kcat/Km)LA ratio more than 5fold for 15-hLO-1 and 3fold for 15-hLO-2, while 12-(S)-hydroperoxyeicosatetraenoic acid affects only the ratio of 15-hLO-1 more than 5fold. In addition, 13-(S)-hydroxyoctadecadienoic acid and 12-(S)-hydroxyeicosatetraenoic acid also affect substrate specificity, indicating that iron oxidation is not responsible for the change in the (kcat/Km)AA/(kcat/Km)LA ratio, residues R402, F414, F352, I417, and M418 are located at the active site and involved in catalysis, presence of a product-activated, allosteric regulatory site for both 15-hLO isozymes, competitive substrate capture experiments, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
15-hLO
-
 Homo sapiens
15-lipoxygenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens