Cloned (Comment) | Organism |
---|---|
expression to high levels, approximately 20% of cellular protein, in a baculovirus/insect cell expression system | Homo sapiens |
General Stability | Organism |
---|---|
repeated freeze thawing especially in the absence of gylcerol results in 60% reduction of specific activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,8,11,14-Eicosatetraynoic acid | inhibits reaction of linoleic acid | Homo sapiens | |
additional information | the enzyme undergoes suicidal inactivation, during fatty acid oxygenation | Homo sapiens | |
nordihydroguaiaretic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
linoleic acid | - |
Homo sapiens | |
0.012 | - |
arachidonic acid | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | addition in a molar excess, more than 50:1 purified protein:Fe2+, leads to a 3fold increase in the specific activity | Homo sapiens | |
Iron | the recombinant enzyme contains a variable amount of iron, often as low as 0.2 mol per mol of enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Homo sapiens |
74727 | - |
x * 74727, electrospray mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 5% carbohydrate | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 5% glycerol, stable for more than 6 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | formation of 15S-hydroxyeicosatetraenoic acid and 12S-hydroxyeicosatetraenoic acid in a ratio of 12:1, double oxygenation products 14R,15S-dihydroxyeicosatetraenoic acid and various 8,15-dihydroxyeicosatetraenoic acids are also produced | ? | |
linoleic acid + O2 | - |
Homo sapiens | 13-hydroxylinoleic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Homo sapiens |
? | x * 74727, electrospray mass spectrometry | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |