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Literature summary for 1.13.11.31 extracted from
- Role of Arg403 for thermostability and catalytic activity of rabbit 12/15-lipoxygenase (2013), Biochim. Biophys. Acta, 1831, 1079-1088.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Oryctolagus cuniculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R403L | a loss of electrostatic interaction between Arg403 and negatively charged amino acid residues of alpha2-helix has only minor impact on protein folding, but partially destabilizes the tertiary structure of the enzyme. Arg403Leu exchange induces strong substrate inhibition. kcat/Km values strongly decreased for linoleic acid and methyl arachidonate but almost unchanged for arachidonic acid compared to wild-type | Oryctolagus cuniculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0082 | - |
arachidonic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus |  |
| 0.0109 | - |
arachidonic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
| 0.0114 | - |
methyl arachidonate | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
| 0.0169 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
| 0.018 | - |
methyl arachidonate | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
| 0.0281 | - |
linoleic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
| linoleic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
| methyl arachidonate + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12/15-lipoxygenase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Oryctolagus cuniculus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 170 | - |
methyl arachidonate | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
| 760 | - |
methyl arachidonate | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
| 830 | - |
linoleic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
| 1410 | - |
arachidonic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
| 1950 | - |
arachidonic acid | pH 7.4, 20°C, mutant R403L | Oryctolagus cuniculus | |
| 2650 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus | |
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