Literature summary for 1.13.11.31 extracted from

Rapp, J.; Xu, S.; Sharp, A.M.; Griffith, W.P.; Kim, Y.W.; Funk, M.O.
EPR spectroscopy and electrospray ionization mass spectrometry reveal distinctive features of the iron site in leukocyte 12-lipoxygenase (2009), Arch. Biochem. Biophys., 490, 50-56.

Search for more literature for 1.13.11.31

Cloned(Commentary)

Cloned (Comment)	Organism
expressed from pET-20b(+) plasmid in Escherichia coli Rosetta 2 (DE3) cells	Sus scrofa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	0.94 atoms of iron per molecule. Loss of the mass of an iron atom from the protein as the pH is lowered from 5 to 4. The iron goes through acid-induced unfolding accompanied by the appearance of an iron-free intermediate that retains a native conformation	Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74700	-	1 * 74700, dynamic light scattering	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P16469	-	-

Purification (Commentary)

Purification (Comment)	Organism
by anion exchange chromatography	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leukocyte	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8	-	purified enzyme	Sus scrofa

Subunits

Subunits	Comment	Organism
monomer	1 * 74700, dynamic light scattering	Sus scrofa

Synonyms

Synonyms	Comment	Organism
12-lipoxygenase	-	Sus scrofa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)