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Literature summary for 1.13.11.30 extracted from

  • Saito, K.; Komamine, A.
    Biosynthesis of stizolobinic acid and stizolobic acid in higher plants (1978), Eur. J. Biochem., 82, 385-392.
    View publication on PubMed
Search for more literature for 1.13.11.30

General Stability

General Stability Organism
DTT stabilizes Stizolobium hassjoo

Inhibitors

Inhibitors Comment Organism Structure
8-Hydroxyquinoline-5-sulfonate 90% inhibition at 0.1 mM Stizolobium hassjoo
Al3+ 95% inhibition at 0.1 mM Stizolobium hassjoo
alpha,alpha'-dipyridyl 70% inhibition at 0.1 mM Stizolobium hassjoo
Co2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
Cu2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
Fe2+ 75% inhibition at 0.1 mM Stizolobium hassjoo
Fe3+ 95% inhibition at 0.1 mM Stizolobium hassjoo
glutathione 70% inhibition at 0.1 mM Stizolobium hassjoo
iodoacetate 55% inhibition at 0.1 mM Stizolobium hassjoo
KCN 85% inhibition at 0.1 mM Stizolobium hassjoo
Mg2+ 65% inhibition at 0.1 mM Stizolobium hassjoo
Mn2+ 95% inhibition at 0.1 mM Stizolobium hassjoo
Mo6+ 80% inhibition at 0.1 mM Stizolobium hassjoo
N-ethylmaleimide 35% inhibition at 0.1 mM Stizolobium hassjoo
Neocuproine 50% inhibition at 0.1 mM Stizolobium hassjoo
o-phenanthroline 90% inhibition at 0.1 mM Stizolobium hassjoo
p-chloromercuribenzoate 70% inhibition at 0.1 mM Stizolobium hassjoo
Salicylaldoxime 65% inhibition at 0.1 mM Stizolobium hassjoo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00026
-
 NADP+
-
 Stizolobium hassjoo
1.67
-
 3,4-dihydroxyphenylalanine
-
 Stizolobium hassjoo

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ activation Stizolobium hassjoo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Stizolobium hassjoo

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxy-L-phenylalanine + O2 Stizolobium hassjoo
-
 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Stizolobium hassjoo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Stizolobium hassjoo

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Stizolobium hassjoo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.031
-
-
 Stizolobium hassjoo

Storage Stability

Storage Stability Organism
-20°C, 0.1 mM DTT, nitrogen atmosphere, stable for 3 months Stizolobium hassjoo
30°C, purified enzyme, half life of 3-5 h Stizolobium hassjoo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?
3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor ?
5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
 Stizolobium hassjoo stizolobinate + NADPH + H+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Stizolobium hassjoo

Cofactor

Cofactor Comment Organism Structure
NAD+ required for reaction, less efficient than NADP+ Stizolobium hassjoo
NADP+ required for reaction Stizolobium hassjoo