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Literature summary for 1.13.11.3 extracted from

  • Buchan, A.; Collier, L.S.; Neidle, E.L.; Moran, M.A.
    Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage (2000), Appl. Environ. Microbiol., 66, 4662-4672.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sagittula stellata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21900
-
 x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits Sagittula stellata
26700
-
 x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits Sagittula stellata

Organism

Organism UniProt Comment Textmining
Roseobacter sp.
-
 isolates GAI-16, S25com04 and IC4, enzyme activity is induced by growth on p-hydroxybenzoate
-
Sagittula stellata
-
 enzyme activity is induced by growth on p-hydroxybenzoate
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.113
-
 activity in E. coli cell extracts expressing the recombinant enzyme Sagittula stellata
0.31
-
 Rosebacter isolate GAI-16, enzyme activity in cell extracts Roseobacter sp.
0.46
-
 enzyme activity in cell extracts Sagittula stellata
0.73
-
 Rosebacter isolate S25com04, enzyme activity in cell extracts Roseobacter sp.
0.88
-
 Rosebacter isolate IC4, enzyme activity in cell extracts Roseobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
 Roseobacter sp. 3-carboxy-cis,cis-muconate
-
 ?
3,4-dihydroxybenzoate + O2
-
 Sagittula stellata 3-carboxy-cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
? x * 21900 + x * 26700, deduced from nucleotide sequences of putative alpha and beta subunits Sagittula stellata