Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | - |
Pseudomonas putida | |
Cl- | - |
Pseudomonas putida | |
F- | - |
Pseudomonas putida | |
I- | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | measurement of Km at different pH values | Pseudomonas putida | |
0.002 | - |
protocatechuate | at pH 8.3, spectrophotometric | Pseudomonas putida | |
0.003 | - |
protocatechuate | at pH 8.3, oxygen electrode | Pseudomonas putida | |
0.07 | - |
O2 | at pH 8.3, spectrophotometric | Pseudomonas putida | |
0.09 | - |
O2 | at pH 8.3, oxygen electrode | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | enzyme contains 4 Fe3+ | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
26500 | - |
alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
190000 | - |
gel filtration | Pseudomonas putida |
202000 | - |
meniscus depletion | Pseudomonas putida |
204000 | - |
sedimentation equilibrium | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
previously classified as Pseudomonas fluorescens PHK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.8 | - |
protocatechuate | oxygen electrode at pH 8.3 | Pseudomonas putida | |
74.7 | - |
protocatechuate | spectrophotometric at pH 8.3 | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas putida |