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Literature summary for 1.13.11.3 extracted from
- Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry (1981), J. Biol. Chem., 256, 12673-12680.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | - |
Pseudomonas putida |  |
| Cl- | - |
Pseudomonas putida | |
| F- | - |
Pseudomonas putida | |
| I- | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | measurement of Km at different pH values | Pseudomonas putida | |
| 0.002 | - |
protocatechuate | at pH 8.3, spectrophotometric | Pseudomonas putida | |
| 0.003 | - |
protocatechuate | at pH 8.3, oxygen electrode | Pseudomonas putida | |
| 0.07 | - |
O2 | at pH 8.3, spectrophotometric | Pseudomonas putida | |
| 0.09 | - |
O2 | at pH 8.3, oxygen electrode | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | enzyme contains 4 Fe3+ | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
| 26500 | - |
alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
| 190000 | - |
gel filtration | Pseudomonas putida |
| 202000 | - |
meniscus depletion | Pseudomonas putida |
| 204000 | - |
sedimentation equilibrium | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
previously classified as Pseudomonas fluorescens PHK | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | alpha,beta, 4 * 23000 + 4 * 26500, SDS-PAGE | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64.8 | - |
protocatechuate | oxygen electrode at pH 8.3 | Pseudomonas putida | |
| 74.7 | - |
protocatechuate | spectrophotometric at pH 8.3 | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Pseudomonas putida |
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