Crystallization (Comment) | Organism |
---|---|
microdialysis of approx. 0.05 ml of enzyme solution, 80 mg/ml against 10 ml volumes of buffered solutions, large single crystals | Brevibacterium fuscum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
protocatechuate | - |
Brevibacterium fuscum | |
0.8 | - |
O2 | - |
Brevibacterium fuscum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 5 Fe atoms per holoenzyme | Brevibacterium fuscum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
40000 | - |
alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
315000 | - |
analytical ultracentrifugation | Brevibacterium fuscum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium fuscum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | carbohydrate contributes less than 0.2% to the mass of the holoenzyme | Brevibacterium fuscum |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium fuscum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Brevibacterium fuscum |
41 | - |
- |
Brevibacterium fuscum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoate + O2 | - |
Brevibacterium fuscum | 3-carboxy-cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | alpha,beta, 5 * 22500 + 5 * 40000, SDS-PAGE | Brevibacterium fuscum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
417 | - |
3,4-dihydroxybenzoate | - |
Brevibacterium fuscum |