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Literature summary for 1.13.11.3 extracted from
- Protocatechuate 3, 4-dioxygenase from Acinetobacter calcoaceticus (1976), Biochemistry, 15, 582-588.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-Dihydroxybenzoate | - |
Acinetobacter calcoaceticus |  |
| 3,4-dihydroxycinnamic acid | - |
Acinetobacter calcoaceticus | |
| 3,4-dihydroxyphenylacetic acid | 0.006 mM, 92% inhibition | Acinetobacter calcoaceticus | |
| 3-Nitrophenol | 0.006 mM, 56% inhibition | Acinetobacter calcoaceticus | |
| 4-hydroxymercuribenzoate | 0.006 mM, 80% inhibition | Acinetobacter calcoaceticus | |
| 4-methylcatechol | 0.006 mM, 59% inhibition | Acinetobacter calcoaceticus | |
| 4-nitrocatechol | 0.006 mM, complete inhibition | Acinetobacter calcoaceticus | |
| alpha-chloro-3,4-dihydroxyacetophenone | - |
Acinetobacter calcoaceticus | |
| catechol | 0-006 mM, 63% inhibition | Acinetobacter calcoaceticus | |
| Co(NO3)2 | - |
Acinetobacter calcoaceticus | |
| CoCl2 | - |
Acinetobacter calcoaceticus | |
| CuCl | - |
Acinetobacter calcoaceticus | |
| CuCl2 | - |
Acinetobacter calcoaceticus | |
| Ferrous ammonium sulfate | - |
Acinetobacter calcoaceticus | |
| additional information | not inhibited by iodoacetamide, 5,5'-dithiobis(2-nitrobenzoate), mercaptoethanol, dithiothreitol, glutathione, o-phenanthroline and H2O2 | Acinetobacter calcoaceticus | |
| Nickel ammonium sulfate | - |
Acinetobacter calcoaceticus | |
| NiCl2 | - |
Acinetobacter calcoaceticus | |
| o-Chloranil | 0.006 mM, 51% inhibition | Acinetobacter calcoaceticus | |
| Protocatechualdehyde | 0.006 mM, 54% inhibition | Acinetobacter calcoaceticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0588 | - |
O2 | - |
Acinetobacter calcoaceticus | |
| 0.0714 | - |
protocatechuate | - |
Acinetobacter calcoaceticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 7.27 g-atoms iron per mol enzyme | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 677000 | - |
sedimentation velocity analysis | Acinetobacter calcoaceticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
derivative of ATCC 14987 | - |
| Acinetobacter calcoaceticus 80-1 | - |
derivative of ATCC 14987 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Acinetobacter calcoaceticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Acinetobacter calcoaceticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8.5, 6 months | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxybenzoate + O2 | - |
Acinetobacter calcoaceticus | 3-carboxy-cis,cis-muconate | - |
? | |
| 3,4-dihydroxybenzoate + O2 | - |
Acinetobacter calcoaceticus 80-1 | 3-carboxy-cis,cis-muconate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 8.5, 1 h, 50% loss of activity | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Acinetobacter calcoaceticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
60°C, 1 h, 50% loss of activity | Acinetobacter calcoaceticus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
4-nitrocatechol | - |
Acinetobacter calcoaceticus | |
| 0.041 | - |
3,4-dihydroxyphenylacetic acid | - |
Acinetobacter calcoaceticus | |
| 0.073 | - |
Protocatechualdehyde | - |
Acinetobacter calcoaceticus | |
| 0.17 | - |
alpha-chloro-3,4-dihydroxyacetophenone | - |
Acinetobacter calcoaceticus | |
| 0.29 | - |
2,5-Dihydroxybenzoate | - |
Acinetobacter calcoaceticus | |
| 0.38 | - |
catechol | - |
Acinetobacter calcoaceticus | |
| 0.44 | - |
4-methylcatechol | - |
Acinetobacter calcoaceticus | |
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