Literature summary for 1.13.11.3 extracted from

Ohlendorf, D.H.; Weber, P.C.; Lipscomb, J.D.
Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa (1987), J. Mol. Biol., 195, 225-227.

Search for more literature for 1.13.11.3

Crystallization (Commentary)

Crystallization (Comment)	Organism
free interface diffusion cell, equal volumes of concentrated enzyme and ammonium sulfate solutions ranging from 37-47% saturation, 4°C, 2.5 A resolution	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	6.6 iron atoms per holoenzyme of 700000 Da	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22280	-	alpha,beta, 2 * 22280 + 2 * 26630, holoenzyme probably contains 6 alpha2,beta2 tetramers, amino acid sequence	Pseudomonas putida
26630	-	alpha,beta, 2 * 22280 + 2 * 26630, holoenzyme probably contains 6 alpha2,beta2 tetramers, amino acid sequence	Pseudomonas putida
590000	-	ultracentrifugation	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	previously classified as Pseudomonas aeruginosa strain ATCC 23975	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,4-dihydroxybenzoate + O2	-	Pseudomonas putida	3-carboxy-cis,cis-muconate	-	?

Subunits

Subunits	Comment	Organism
More	quarternary structure	Pseudomonas putida
tetramer	alpha,beta, 2 * 22280 + 2 * 26630, holoenzyme probably contains 6 alpha2,beta2 tetramers, amino acid sequence	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)