Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-butanol | 5% v/v, 2.9fold activation | Sus scrofa | |
1-propanol | 10% v/v, 2.1fold activation | Sus scrofa | |
acetone | 10% v/v, 5.7fold activation | Sus scrofa | |
acetonitrile | 10% v/v, 3.6fold activation | Sus scrofa | |
Cyclohexanol | 5% v/v, 4.4fold activation | Sus scrofa | |
dioxane | 10% v/v, 2.8fold activation | Sus scrofa | |
ethanol | 10% v/v, 2fold activation | Sus scrofa | |
ether | 10% v/v, 2.6fold activation | Sus scrofa | |
methanol | 10% v/v, 1.6fold activation | Sus scrofa | |
methyl-cellosolve | 10% v/v, 2.2fold activation | Sus scrofa | |
tetrahydrofuran | 10% v/v, 6.7fold activation | Sus scrofa |
General Stability | Organism |
---|---|
stable to freezing and thawing | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value is the same at 25°C and at 38°C | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 0.4 atoms of Fe per 89000 Da enzyme. Removal of copper does not correlate with loss of activity | Sus scrofa | |
Iron | 0.9 atoms of Fe per 89000 Da enzyme. Removal of iron correlates with loss of activity | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, nonidentical subunits, SDS-PAGE | Sus scrofa |
50000 | 54000 | gel filtration | Sus scrofa |
89000 | - |
equilibrium ultracentrifugation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
hog | - |
Oxidation Stability | Organism |
---|---|
routinely stored in presence of O2 | Sus scrofa |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.6 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, concentrated solution, 1 mg/ml, pH 6.0, stable for 6 months or more | Sus scrofa |
-20°C, on long-term storage the enzyme forms polymers, reversal by thiols | Sus scrofa |
4°C, dilute solutions, 0.1 mg/ml, 100 mM sodium acetate buffer, PH 6.0, stable for up to 2 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Sus scrofa | homogentisate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, nonidentical subunits, SDS-PAGE | Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzymatic rate increases approximately 5fold for every 10°C increase, Km-value is the same at 25°C and at 38°C | Sus scrofa |