Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
about 40000 Da, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quercetin + O2 | Bacillus subtilis | - |
2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
quercetin + O2 | Bacillus subtilis 168 | - |
2-protocatechoylphloroglucinolcarboxylate + CO | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 168 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by ammonium sulfate precipitation and DEAE Toyopearl column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
native enzyme, using quercetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
0.0276 | - |
recombinant enzyme, using fisetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
1.87 | - |
recombinant enzyme, using tamarixetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
2.27 | - |
recombinant enzyme, using quercetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
2.49 | - |
recombinant enzyme, using galangin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fisetin + O2 | 1.22% activity compared to quercetin | Bacillus subtilis | 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO | - |
? | |
fisetin + O2 | 1.22% activity compared to quercetin | Bacillus subtilis 168 | 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO | - |
? | |
galangin + O2 | 110% activity compared to quercetin | Bacillus subtilis | 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO | - |
? | |
galangin + O2 | 110% activity compared to quercetin | Bacillus subtilis 168 | 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO | - |
? | |
quercetin + O2 | - |
Bacillus subtilis | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
quercetin + O2 | 100% activity | Bacillus subtilis | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
quercetin + O2 | - |
Bacillus subtilis 168 | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
quercetin + O2 | 100% activity | Bacillus subtilis 168 | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
tamarixetin + O2 | 82.4% activity compared to quercetin | Bacillus subtilis | 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO | - |
? | |
tamarixetin + O2 | 82.4% activity compared to quercetin | Bacillus subtilis 168 | 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO | - |
? |
Synonyms | Comment | Organism |
---|---|---|
QdoI | - |
Bacillus subtilis |