Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | activates, restores the inhibition by Fe2+ | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | non preincubated protein-B: 76% inhibition at 1 mM, with cysteine preincubated protein-B: no inhibition | Rattus norvegicus | |
EDTA | non preincubated protein-B: 91% inhibition at 1 mM, with cysteine preincubated protein-B: 51% inhibition at 1 mM | Rattus norvegicus | |
Fe2+ | inhibits the enzyme activity of both preactivated and non-preactivated protein-B | Rattus norvegicus | |
o-phenanthroline | non-preincubated protein-B: 91% inhibition at 1 mM, with cysteine preincubated protein-B: 67% inhibition at 1 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using acid treatment, ammonium sulfate fractionation and column chromatography with DEAE-cellulose. The purified enzyme is composed of two distinct proteins, it appears that one of them is a catalytic protein named protein-B having tightly bound iron as a prosthetic group, while the other is either a modifier or activating protein named protein-A. Protein-B is found to exist in both an active and an inactive form | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
additional information | glutathione, dithiothreitol and cystine do not serve as substrates | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | liver enzyme is composed of 2 distinct proteins: 1. protein-B, tightly bound iron as prosthetic group, 2. protein A, modifier or activating protein | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of NAD, NADP, NADH or NADPH for the enzymic activity of protein-B. The enzymic activity of protein-B alone is extremely low, protein-A alone does not exhibit catalytic activity, however a significant activity is observed in the presence of both fractions, requirement of protein-A for the catalytic activity of protein-B | Rattus norvegicus |