Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-vinylcatechol | C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol | Pseudomonas putida | |
additional information | C23O activity (TodE) is unaffected by styrene, toluene, and 3-methylcatechol | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and Prepease nickel resin chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-vinylcatechol + O2 | - |
Pseudomonas putida | ? | - |
? | |
catechol + O2 | - |
Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C23o | - |
Pseudomonas putida |
TodE | - |
Pseudomonas putida |
Organism | Comment | Expression |
---|---|---|
Pseudomonas putida | C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol | down |
Pseudomonas putida | C23O activity (TodE) is is unaffected by styrene, toluene, and 3-methylcatechol | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of TodE and the subsequent accumulation of 3-vinylcatechol results in toxicity and cell death | Pseudomonas putida |
physiological function | overexpressing TodE is necessary to allow Pseudomonas putida strain F1 to grow on styrene | Pseudomonas putida |