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Literature summary for 1.13.11.2 extracted from

  • George, K.W.; Kagle, J.; Junker, L.; Risen, A.; Hay, A.G.
    Growth of Pseudomonas putida F1 on styrene requires increased catechol-2,3-dioxygenase activity, not a new hydrolase (2011), Microbiology, 157, 89-98.
    View publication on PubMed
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
3-vinylcatechol C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol Pseudomonas putida
additional information C23O activity (TodE) is unaffected by styrene, toluene, and 3-methylcatechol Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation and Prepease nickel resin chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-vinylcatechol + O2
-
 Pseudomonas putida ?
-
 ?
catechol + O2
-
 Pseudomonas putida 2-hydroxymuconate semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
C23o
-
 Pseudomonas putida
TodE
-
 Pseudomonas putida

Expression

Organism Comment Expression
Pseudomonas putida C23O activity (TodE) is almost completely abrogated by exposure to 0.2 mM 3-vinylcatechol down
Pseudomonas putida C23O activity (TodE) is is unaffected by styrene, toluene, and 3-methylcatechol additional information

General Information

General Information Comment Organism
malfunction inactivation of TodE and the subsequent accumulation of 3-vinylcatechol results in toxicity and cell death Pseudomonas putida
physiological function overexpressing TodE is necessary to allow Pseudomonas putida strain F1 to grow on styrene Pseudomonas putida